可适应的多聚（乙二醇）微球，具有混合模式降解能力。
Adaptable poly(ethylene glycol) microspheres capable of mixed-mode degradation.
机构信息
Department of Biomedical Engineering, University of Wisconsin Madison, Wisconsin Institutes for Medical Research, 1111 Highland Ave., Madison, WI 53705, USA.
出版信息
Acta Biomater. 2013 Dec;9(12):9270-80. doi: 10.1016/j.actbio.2013.08.011. Epub 2013 Aug 17.
Abstract
A simple, degradable poly(ethylene glycol) (PEG) microsphere system formed from a water-in-water emulsion process is presented. Microsphere network degradation and erosion were controlled by adjusting the number of hydrolytically labile sites, by varying the PEG molecular weight, and by adjusting the emulsion conditions. Microsphere size was also controllable by adjusting the polymer formulation. Furthermore, it is demonstrated that alternative degradation and erosion mechanisms, such as proteolytic degradation, can be incorporated into PEG microspheres, resulting in mixed-mode degradation. Owing to the adaptability of this approach, it may serve as an attractive option for emerging tissue engineering, drug delivery and gene delivery applications.
摘要
本文介绍了一种简单的、可降解的聚乙二醇（PEG）微球体系，通过水包水乳液工艺形成。通过调整水解不稳定部位的数量、PEG 分子量和乳液条件来控制微球网络的降解和侵蚀。通过调整聚合物配方还可以控制微球的大小。此外，还证明了可以将替代的降解和侵蚀机制（如蛋白水解降解）纳入 PEG 微球中，从而导致混合模式降解。由于这种方法的适应性，它可能成为新兴的组织工程、药物输送和基因输送应用的一个有吸引力的选择。
相似文献
1
Adaptable poly(ethylene glycol) microspheres capable of mixed-mode degradation.可适应的多聚（乙二醇）微球，具有混合模式降解能力。
Acta Biomater. 2013 Dec;9(12):9270-80. doi: 10.1016/j.actbio.2013.08.011. Epub 2013 Aug 17.
2
Raloxifene-/raloxifene-poly(ethylene glycol) conjugate-loaded microspheres: A novel strategy for drug delivery to bone forming cells.载雷洛昔芬/雷洛昔芬-聚乙二醇轭合物的微球：一种向成骨细胞递药的新策略。
Int J Pharm. 2016 Aug 20;510(1):168-83. doi: 10.1016/j.ijpharm.2016.06.053. Epub 2016 Jun 23.
3
Poly(ethylene glycol) methacrylate hydrolyzable microspheres for transient vascular embolization.用于短暂血管栓塞的聚甲基丙烯酸乙二醇酯可水解微球
Acta Biomater. 2014 Mar;10(3):1194-205. doi: 10.1016/j.actbio.2013.11.028. Epub 2013 Dec 7.
4
Cross-linking and degradation of step-growth hydrogels formed by thiol-ene photoclick chemistry.巯基-烯光点击化学形成的逐步增长水凝胶的交联和降解。
Biomacromolecules. 2012 Jul 9;13(7):2003-12. doi: 10.1021/bm300752j. Epub 2012 Jun 22.
5
An approach to modulate degradation and mesenchymal stem cell behavior in poly(ethylene glycol) networks.一种调节聚乙二醇网络中降解及间充质干细胞行为的方法。
Biomacromolecules. 2008 Mar;9(3):842-9. doi: 10.1021/bm701179s. Epub 2008 Feb 21.
6
In vitro degradation and release profiles for poly-dl-lactide-poly(ethylene glycol) microspheres containing human serum albumin.含人血清白蛋白的聚-dl-丙交酯-聚（乙二醇）微球的体外降解和释放曲线
J Control Release. 2001 Apr 2;71(2):165-73. doi: 10.1016/s0168-3659(01)00210-3.
7
Control of silk microsphere formation using polyethylene glycol (PEG).使用聚乙二醇（PEG）控制丝微球的形成。
Acta Biomater. 2016 Jul 15;39:156-168. doi: 10.1016/j.actbio.2016.05.019. Epub 2016 May 12.
8
The effect of poly(ethylene glycol)-poly(D,L-lactic acid) diblock copolymers on peptide acylation.聚（乙二醇）-聚（D，L-乳酸）二嵌段共聚物对肽酰化的影响。
J Control Release. 2002 Apr 23;80(1-3):157-68. doi: 10.1016/s0168-3659(02)00020-2.
9
Stabilization and controlled release of bovine serum albumin encapsulated in poly(D, L-lactide) and poly(ethylene glycol) microsphere blends.聚（D，L-丙交酯）与聚乙二醇微球共混物中包封的牛血清白蛋白的稳定化及控释
Pharm Res. 2001 Jun;18(6):878-85. doi: 10.1023/a:1011009117586.
10
Designing Visible Light-Cured Thiol-Acrylate Hydrogels for Studying the HIPPO Pathway Activation in Hepatocellular Carcinoma Cells.设计用于研究肝癌细胞中HIPPO信号通路激活的可见光固化硫醇-丙烯酸酯水凝胶
Macromol Biosci. 2016 Apr;16(4):496-507. doi: 10.1002/mabi.201500361. Epub 2015 Dec 28.
引用本文的文献
1
Microparticle Hydrogel Material Properties Emerge from Mixing-Induced Homogenization in a Poly(ethylene glycol) and Dextran Aqueous Two-Phase System.微粒水凝胶材料特性源自聚乙二醇和右旋糖酐水两相体系中混合诱导的均匀化过程。
Macromolecules. 2023 Oct 30;56(21):8518-8528. doi: 10.1021/acs.macromol.3c00557. eCollection 2023 Nov 14.
2
Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells.用于 3D ECM 筛选和原代人肝星状细胞异质微环境培养的组合微凝胶。
Adv Sci (Weinh). 2024 Apr;11(15):e2303128. doi: 10.1002/advs.202303128. Epub 2024 Feb 13.
3
Microfluidic Platform for Microparticle Fabrication and Release of a Cathepsin Inhibitor.用于微颗粒制造和释放组织蛋白酶抑制剂的微流控平台。
Tissue Eng Part C Methods. 2023 Aug;29(8):361-370. doi: 10.1089/ten.TEC.2023.0015.
4
Microfluidic Chip Device for Mixing and Fabrication of Hydrogel Microspheres via Michael-Type Addition.微流控芯片装置通过迈克尔加成法混合和制备水凝胶微球。
Langmuir. 2021 Oct 12;37(40):11793-11803. doi: 10.1021/acs.langmuir.1c01739. Epub 2021 Oct 1.
5
Controlling Structure with Injectable Biomaterials to Better Mimic Tissue Heterogeneity and Anisotropy.用可注射生物材料控制结构以更好地模拟组织异质性和各向异性。
Adv Healthc Mater. 2021 Jun;10(11):e2002221. doi: 10.1002/adhm.202002221. Epub 2021 May 5.
6
Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids.水凝胶微粒与三维肝祖细胞球体的整合
Front Bioeng Biotechnol. 2020 Jul 21;8:792. doi: 10.3389/fbioe.2020.00792. eCollection 2020.
7
Printing Therapeutic Proteins in 3D using Nanoengineered Bioink to Control and Direct Cell Migration.使用纳米工程生物墨水 3D 打印治疗性蛋白以控制和引导细胞迁移。
Adv Healthc Mater. 2019 Jun;8(11):e1801553. doi: 10.1002/adhm.201801553. Epub 2019 May 8.
8
Core-shell microparticles for protein sequestration and controlled release of a protein-laden core.用于蛋白质螯合和载有蛋白质的核心的控释的核壳微粒。
Acta Biomater. 2017 Jul 1;56:91-101. doi: 10.1016/j.actbio.2016.12.042. Epub 2016 Dec 21.
9
Stimuli-Responsive Polymeric Systems for Controlled Protein and Peptide Delivery: Future Implications for Ocular Delivery.用于蛋白质和肽可控递送的刺激响应性聚合物系统：对眼部递送的未来影响。
Molecules. 2016 Jul 30;21(8):1002. doi: 10.3390/molecules21081002.
10
Methods for Generating Hydrogel Particles for Protein Delivery.用于蛋白质递送的水凝胶颗粒生成方法。
Ann Biomed Eng. 2016 Jun;44(6):1946-58. doi: 10.1007/s10439-016-1637-z. Epub 2016 May 9.
本文引用的文献
1
A Versatile Synthetic Extracellular Matrix Mimic via Thiol-Norbornene Photopolymerization.通过巯基-降冰片烯光聚合制备多功能仿生细胞外基质
Adv Mater. 2009 Dec 28;21(48):5005-5010. doi: 10.1002/adma.200901808. Epub 2009 Oct 7.
2
Regulating specific growth factor signaling using immobilized branched ligands.利用固定化支化配体调节特定生长因子信号。
Adv Healthc Mater. 2012 Jul;1(4):457-60. doi: 10.1002/adhm.201200077. Epub 2012 May 11.
3
Specific VEGF sequestering and release using peptide-functionalized hydrogel microspheres.使用肽功能化水凝胶微球特异性隔离和释放 VEGF。
Biomaterials. 2012 Apr;33(12):3475-84. doi: 10.1016/j.biomaterials.2012.01.032. Epub 2012 Feb 7.
4
Influence of PEG in PEG-PLGA microspheres on particle properties and protein release.聚乙二醇（PEG）在 PEG-PLGA 微球中对颗粒性质和蛋白质释放的影响。
Eur J Pharm Biopharm. 2012 May;81(1):57-63. doi: 10.1016/j.ejpb.2012.01.009. Epub 2012 Jan 28.
5
Modular poly(ethylene glycol) scaffolds provide the ability to decouple the effects of stiffness and protein concentration on PC12 cells.模块化聚乙二醇支架提供了分离刚度和蛋白质浓度对 PC12 细胞影响的能力。
Acta Biomater. 2011 Nov;7(11):3841-9. doi: 10.1016/j.actbio.2011.06.054. Epub 2011 Jul 13.
6
Adaptive micro and nanoparticles: temporal control over carrier properties to facilitate drug delivery.自适应微纳米颗粒：通过时间控制载体性能以促进药物传递。
Adv Drug Deliv Rev. 2011 Nov;63(14-15):1247-56. doi: 10.1016/j.addr.2011.05.004. Epub 2011 May 13.
7
Facile formation of dynamic hydrogel microspheres for triggered growth factor delivery.用于触发生长因子递送的动态水凝胶微球的简便形成方法。
Acta Biomater. 2011 Mar;7(3):975-85. doi: 10.1016/j.actbio.2010.10.026. Epub 2010 Oct 26.
8
The formation of protein concentration gradients mediated by density differences of poly(ethylene glycol) microspheres.由聚乙二醇微球密度差异介导的蛋白质浓度梯度的形成。
Biomaterials. 2010 Nov;31(33):8642-50. doi: 10.1016/j.biomaterials.2010.07.085. Epub 2010 Aug 16.
9
Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds with tunable degradation and mechanical properties.可水解的聚乙二醇水凝胶支架，具有可调降解和机械性能。
Biomacromolecules. 2010 May 10;11(5):1348-57. doi: 10.1021/bm100137q.
10
Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering.用于组织工程的聚乙二醇水凝胶的生物活性修饰。
Biomaterials. 2010 Jun;31(17):4639-56. doi: 10.1016/j.biomaterials.2010.02.044. Epub 2010 Mar 19.
Zotero 插件