Pathophysiology Program, Faculty of Medicine, University of Chile, Chile.
Biol Res. 2013;46(2):207-13. doi: 10.4067/S0716-97602013000200013.
Oxidant/antioxidant imbalance has been reported in some infectious diseases, including community-acquired pneumonia (CAP). The aim was to assess the antioxidant status in adults with CAP and its relationship with clinical severity at admission. Fifty-nine patients with CAP were enrolled and categorized at admission by the FINE score, from July 2010 to October 2012. In the same period 61 controls were enrolled. Plasma samples were obtained at admission for determination of the ferric reducing ability of plasma (FRAP) and lipid peroxidation (8-isoprostane). Erythrocyte reduced (GSH)/oxidized (GSSG) glutathione, malondialdehyde (MDA) and antioxidant enzyme activity were assessed. Antioxidant status in adults with CAP represented by FRAP and the GSH/GSSG ratio were 16.8% (p=0.03) and 39.7% (p=0.04) lower than control values, respectively. In addition, FRAP values showed a positive correlation with GSH/GSSG ratio (r=0.852; p<0.02; n=59). The CAP group showed greater lipid peroxidation in both plasma and erythrocytes. The FINE score correlated negatively with FRAP (r= -0.718; p<0.05; n=59) and positively with MDA and F2 isoprostane levels (r=0.673; p<0.05; n=59; r=0.892; p<0.01; n=59, respectively). Antioxidant status alterations correlated with clinical severity. The FRAP assay and lipid peroxidation biomarkers may provide a useful parameter for estimating the severity and the clinical outcome of patients with CAP.
氧化剂/抗氧化剂失衡已在一些传染病中报道，包括社区获得性肺炎（CAP）。目的是评估成人 CAP 的抗氧化状态及其与入院时临床严重程度的关系。2010 年 7 月至 2012 年 10 月，纳入 59 例 CAP 患者，并根据 FINE 评分进行入院分类。同期纳入 61 例对照。入院时采集血浆样本，测定血浆铁还原能力（FRAP）和脂质过氧化（8-异前列腺素）。评估红细胞还原型（GSH）/氧化型（GSSG）谷胱甘肽、丙二醛（MDA）和抗氧化酶活性。以 FRAP 和 GSH/GSSG 比值代表的 CAP 成人抗氧化状态分别比对照组低 16.8%（p=0.03）和 39.7%（p=0.04）。此外，FRAP 值与 GSH/GSSG 比值呈正相关（r=0.852；p<0.02；n=59）。CAP 组在血浆和红细胞中均显示出更大的脂质过氧化。FINE 评分与 FRAP 呈负相关（r=-0.718；p<0.05；n=59），与 MDA 和 F2 异前列腺素水平呈正相关（r=0.673；p<0.05；n=59；r=0.892；p<0.01；n=59）。抗氧化状态的改变与临床严重程度相关。FRAP 测定和脂质过氧化生物标志物可能为评估 CAP 患者的严重程度和临床结局提供有用的参数。
