Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, PR China.
J Hazard Mater. 2013 Oct 15;261:325-31. doi: 10.1016/j.jhazmat.2013.07.022. Epub 2013 Jul 20.
Chlorination of the secondary effluent from domestic wastewater treatment plants (WWTP) is suspicious of toxic effects due to the formation of trihalomethanes (THMs). In order to clarify this mechanism, samples were collected from the inlet and outlet of a WWTP, and a series of analyses were conducted on the relationship between THMs formation and the toxicity of the secondary effluent after chlorination. By three-dimensional fluorescence spectrometry it was clarified that the biological wastewater treatment process was accompanied with the formation of humic- and fulvic-like substances which were typical THMs precursors as shown by their high thihalomethane formation potential (THMFP) as well as high specific ultraviolet absorbance (SUVA). As a result of bioassay using a freshwater luminescent bacterium Vibrio-qinghaiensis sp.-Q67, a linear relationship was obtained between the concentration of THMs after chlorinating the effluent and its toxicity expressed as the inhibition of the luminescent light. The effective THMs concentration corresponding to a 50% inhibition was 318 μg/L at a chlorine dose of 19.6 mg/L as Cl2. THMs formation may be the direct reason for the increase of toxicity in the secondary effluent when chlorine was used as disinfectant.
生活污水处理厂（WWTP）二级出水的氯化物由于三卤甲烷（THMs）的形成而被怀疑具有毒性作用。为了阐明这一机制，从 WWTP 的进出口采集了水样，并对氯化后二级出水 THMs 形成与毒性之间的关系进行了一系列分析。通过三维荧光光谱法，明确了生物废水处理过程伴随着腐殖质和富里酸样物质的形成，这些物质是典型的 THMs 前体，其具有高的三卤甲烷生成潜能（THMFP）和高的特定紫外吸光度（SUVA）。采用淡水发光细菌 Vibrio-qinghaiensis sp.-Q67 进行生物测定，结果表明，在氯投加量为 19.6mg/L 时，出水经氯化后 THMs 的浓度与其毒性（以发光抑制率表示）之间呈线性关系。当氯作为消毒剂时，THMs 的形成可能是二级出水中毒性增加的直接原因。有效 THMs 浓度对应于 318μg/L，抑制率为 50%。
