Mycothèque de l'Université catholique de Louvain (BCCM/MUCL), Earth and Life Institute, Mycology, Université catholique de Louvain, Croix du Sud 2, bte L7.05.06, 1348 Louvain-la-Neuve, Belgium.
Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, C.P. 486, Belo Horizonte, MG 31270-901, Brazil.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3896-3903. doi: 10.1099/ijs.0.055897-0. Epub 2013 Aug 19.
A novel yeast species was found repeatedly and in high cell densities in underground-nesting stingless bees of the species Melipona quinquefasciata and their provisions in northern Minas Gerais (Brazil). One additional strain was isolated from bee-collected pollen in Cuba. Phylogenetic analyses based on rRNA gene sequences (D1/D2 large subunit gene and internal transcribed spacer) indicated that the novel species belongs to the Starmerella clade and is most closely related to Candida (iter. nom. Starmerella) apicola. Growth reactions on carbon and nitrogen sources were typical of those observed in related species of the Starmerella clade. PCR-fingerprinting with mini- and microsatellite specific primers allowed the distinction of the novel species from Candida apicola, Candida bombi and a yet undescribed species represented by strain CBS 4353. On the basis of phylogenetic relationships, the novel species is assigned to the genus Starmerella despite the failure to observe sexual reproduction after extensive mating tests. We propose the name Starmerella neotropicalis f. a., sp. nov. (Mycobank MB 804285) and designate UFMG PST 09(T) ( = MUCL 53320(T) = CBS 12811(T)) as the type strain.
在巴西米纳斯吉拉斯州北部地下筑巢的无刺蜜蜂及其蜜囊中，反复且以高密度存在一种新型酵母。另外一个菌株是从古巴蜜蜂采集的花粉中分离出来的。基于 rRNA 基因序列（D1/D2 大亚基基因和内部转录间隔区）的系统发育分析表明，该新型酵母物种属于 Starmerella 进化枝，与 Candida（暂定名 Starmerella）apicola 关系最为密切。对碳源和氮源的生长反应与 Starmerella 进化枝中相关物种的观察结果一致。使用 mini- 和 microsatellite 特异性引物的 PCR 指纹图谱可将新型物种与 Candida apicola、Candida bombi 和一个尚未描述的由 CBS 4353 代表的物种区分开来。基于系统发育关系，尽管在广泛的交配试验后未能观察到有性繁殖，该新型物种仍被分配到 Starmerella 属中。我们提出了 Starmerella neotropicalis f. a., sp. nov.（Mycobank MB 804285）的名称，并指定 UFMG PST 09(T)（ = MUCL 53320(T) = CBS 12811(T)）为模式株。
