肠球菌 saccharolyticus 亚种。台湾亚种，从西兰花中分离得到。
Enterococcus saccharolyticus subsp. taiwanensis subsp. nov., isolated from broccoli.
机构信息
Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Road, Gui-Shan Township, Taoyuan County 333, Taiwan.
The Institute of Enology and Viticulture, Yamanashi University, 1-13-1, Kitashin, Kofu, Yamanashi 400-0005, Japan.
出版信息
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4691-4697. doi: 10.1099/ijs.0.052761-0. Epub 2013 Aug 19.
A coccal strain isolated from fresh broccoli was initially identified as Enterococcus saccharolyticus; however, molecular identification and phenotypic traits did not support this identification. DNA-DNA hybridization with the type strain of E. saccharolyticus (76.4 % relatedness), DNA G+C content (35.7 mol%), phylogenetic analysis based on 16S rRNA, pheS and rpoA gene sequences, rep-PCR fingerprinting and profiles of cellular fatty acids, whole-cell proteins and enzyme activities, together with carbohydrate metabolism characteristics, indicated that this strain is distinct and represents a novel subspecies, for which the name Enterococcus saccharolyticus subsp. taiwanensis subsp. nov. is proposed. The type strain is 812(T) ( = NBRC 109476(T) = BCRC 80575(T)). Furthermore, we present an emended description of Enterococcus saccharolyticus and proposal of Enterococcus saccharolyticus subsp. saccharolyticus subsp. nov. (type strain ATCC 43076(T) = CCUG 27643(T) = CCUG 33311(T) = CIP 103246(T) = DSM 20726(T) = JCM 8734(T) = LMG 11427(T) = NBRC 100493(T) = NCIMB 702594(T)).
从新鲜西兰花中分离出的球形菌株最初被鉴定为解糖肠球菌；然而，分子鉴定和表型特征不支持这种鉴定。与解糖肠球菌的模式株（76.4%的相关性）、DNA G+C 含量（35.7mol%）、基于 16S rRNA、pheS 和 rpoA 基因序列的系统发育分析、rep-PCR 指纹图谱和细胞脂肪酸、全细胞蛋白和酶活性图谱以及碳水化合物代谢特征的 DNA-DNA 杂交表明，该菌株是独特的，代表了一个新的亚种，因此提议将其命名为解糖肠球菌亚种。模式株为 812(T)（=NBRC 109476(T)=BCRC 80575(T)）。此外，我们对解糖肠球菌进行了修订描述，并提议将解糖肠球菌亚种。解糖亚种 nov.（模式株 ATCC 43076(T)=CCUG 27643(T)=CCUG 33311(T)=CIP 103246(T)=DSM 20726(T)=JCM 8734(T)=LMG 11427(T)=NBRC 100493(T)=NCIMB 702594(T)）。
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