Departamento de Biotecnología de Alimentos, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Padre García Tejero 4, 41012 Sevilla, Spain.
Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 1052, 41080 Sevilla, Spain.
Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2534-2539. doi: 10.1099/ijs.0.062208-0. Epub 2014 May 8.
Six strains of a hitherto unknown, Gram-stain-positive coccus were recovered from samples of Spanish-style green-olive fermentations. The 16S rRNA gene sequences from these isolates shared 98.7% and 98.5% of their nucleotide positions with those from Enterococcus saccharolyticus subsp. taiwanensis 812(T) and from E. saccharolyticus subsp. saccharolyticus ATCC 43076(T), respectively. The sequence of the rpoA gene in the isolates was 95% similar to that of E. saccharolyticus CECT 4309(T) ( = ATCC 43076(T)). The 16S rRNA and rpoA gene phylogenies revealed that the isolates grouped in a statistically well-supported cluster separate from E. saccharolyticus. Enzyme activity profiles as well as fermentation patterns differentiated the novel bacteria from other members of the Enterococcus genus. Finally, phenotypic, genotypic and phylogenetic data supported the identification of a novel species of the genus Enterococcus, for which the name Enterococcus olivae sp. nov. is proposed. The type strain is IGG16.11(T) ( = CECT 8063(T) = DSM 25431(T)).
从西班牙式绿橄榄发酵样品中分离到六株未知的革兰氏阳性球菌。这些分离株的 16S rRNA 基因序列与肠球菌 saccharolyticus 亚种 taiwanensis 812(T) 和肠球菌 saccharolyticus 亚种 saccharolyticus ATCC 43076(T) 的核苷酸位置分别共享 98.7%和 98.5%。分离株的 rpoA 基因序列与肠球菌 saccharolyticus CECT 4309(T)（=ATCC 43076(T)）的相似度为 95%。16S rRNA 和 rpoA 基因系统发育分析表明，这些分离株与肠球菌属的其他成员形成了一个在统计学上支持的聚类。酶活性谱和发酵模式将新型细菌与肠球菌属的其他成员区分开来。最后，表型、基因型和系统发育数据支持鉴定出一种新型肠球菌属细菌，将其命名为 Enterococcus olivae sp. nov.。模式菌株为 IGG16.11(T)（=CECT 8063(T)=DSM 25431(T)）。
