Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium.
Vakgroep Veterinaire Volksgezondheid en Voedselveiligheid, Faculteit Diergeneeskunde, Universiteit Gent, Gent, Belgium.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4707-4718. doi: 10.1099/ijs.0.048900-0. Epub 2013 Aug 19.
Analysis of partial gyrB gene sequences revealed six taxa in a group of 17 Burkholderia glathei-like isolates which were further examined by (GTG)5-PCR fingerprinting, 16S rRNA gene sequence analysis, DNA-DNA hybridizations, determination of the DNA G+C content, whole-cell fatty acid analysis and an analysis of cell and colony morphology and more than 180 biochemical characteristics. The results demonstrated that one taxon consisting of three human clinical isolates represented Burkholderia zhejiangensis, a recently described methyl-parathion-degrading bacterium isolated from a wastewater-treatment system in China. The remaining taxa represented five novel species isolated from soil or rhizosphere soil samples, and could be distinguished by both genotypic and phenotypic characteristics. We therefore propose to formally classify these bacteria as Burkholderia humi sp. nov. (type strain, LMG 22934(T) = CCUG 63059(T)), Burkholderia choica sp. nov. (type strain, LMG 22940(T) = CCUG 63063(T)), Burkholderia telluris sp. nov. (type strain, LMG 22936(T) = CCUG 63060(T)), Burkholderia udeis sp. nov. (type strain, LMG 27134(T) = CCUG 63061(T)) and Burkholderia terrestris sp. nov. (type strain, LMG 22937(T) = CCUG 63062(T)).
对部分 gyrB 基因序列的分析揭示，在一组 17 个伯克霍尔德氏菌 glathei 样分离株中存在 6 个分类群，这些分类群进一步通过（GTG）5-PCR 指纹图谱分析、16S rRNA 基因序列分析、DNA-DNA 杂交、DNA G+C 含量测定、全细胞脂肪酸分析以及细胞和菌落形态分析和 180 多项生化特征分析进行了研究。结果表明，由 3 个人类临床分离株组成的一个分类群代表了一种新描述的从中国废水处理系统中分离出的甲基对硫磷降解细菌，称为浙江伯克霍尔德氏菌。其余的分类群代表了从土壤或根际土壤样本中分离出的 5 种新型物种，可通过基因型和表型特征来区分。因此，我们建议将这些细菌正式分类为 Humibacterium humi sp. nov.（模式株，LMG 22934（T）=CCUG 63059（T））、Choibacterium choica sp. nov.（模式株，LMG 22940（T）=CCUG 63063（T））、Tellurobacterium telluris sp. nov.（模式株，LMG 22936（T）=CCUG 63060（T））、Udeibacterium udeis sp. nov.（模式株，LMG 27134（T）=CCUG 63061（T））和 Terrabacter terrestris sp. nov.（模式株，LMG 22937（T）=CCUG 63062（T））。
