Department of Biology, Jeju National University, Jeju, 63243, Republic of Korea.
J Microbiol. 2018 May;56(5):312-316. doi: 10.1007/s12275-018-8034-2. Epub 2018 May 2.
A rod-shaped, round and white colony-forming strain AD18 was isolated from the soil on Halla mountain in Jeju Island, Republic of Korea. Comparative analysis of 16S rRNA gene sequence revealed that this strain was closely related to Burkholderia oklahomensis C6786 (98.8%), Burkholderia thailandensis KCTC 23190 (98.5%). DNA-DNA relatedness (14.6%) indicated that the strain AD18 represents a distinct species that is separate from B. oklahomensis C6786. The isolate grew at pH 5.0-9.0 (optimum, pH 7.0), 0-3% (w/v) NaCl (optimum, 0%), and temperature 10-40°C (optimum 35°C). The sole quinone of the strain was Q-8, and the predominant fatty acids were C, C cyclo, and C cyclo ω8c. The genomic DNA G + C content of AD18T was 65.6 mol%. Based on these findings, strain AD18 is proposed to be a novel species in the genus Burkholderia, for which the name Burkholderia alba sp. nov. is proposed (= KCCM 43268 = JCM 32403). The type strain is AD18.
一株杆状、圆形、白色的集落形成菌株 AD18 从韩国济州岛汉拿山的土壤中分离得到。16S rRNA 基因序列的比较分析表明，该菌株与奥克拉荷马伯克霍尔德菌 C6786（98.8%）、泰国伯克霍尔德菌 KCTC 23190（98.5%）密切相关。DNA-DNA 同源性（14.6%）表明菌株 AD18 代表了一个与奥克拉荷马伯克霍尔德菌 C6786 不同的独特物种。该分离株在 pH5.0-9.0（最佳，pH7.0）、0-3%（w/v）NaCl（最佳，0%）和 10-40°C（最佳 35°C）下生长。该菌株的唯一醌类物质为 Q-8，主要脂肪酸为 C、C 环和 C 环 ω8c。AD18T 的基因组 DNA G+C 含量为 65.6mol%。基于这些发现，菌株 AD18 被提议为伯克霍尔德菌属的一个新种，命名为 Burkholderia alba sp. nov.（=KCCM 43268=JCM 32403）。模式菌株为 AD18。
