Karamifar Kasra, Khayat Akbar, Mogharrabi Sara, Rajaei Yasaman, Saghiri Mohammad Ali
Department of Endodontics, Dental School, Shiraz Branch, Islamic Azad University, Shiraz, Iran ; Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Saudi Dent J. 2012 Jul;24(3-4):157-62. doi: 10.1016/j.sdentj.2012.03.002. Epub 2012 May 12.
The purpose of this study was to evaluate the effect of gravity and capillarity on penetration of human salivary bacteria into the entire length of obturated root canals, and to demonstrate the dye penetration configuration.
Fifty single-rooted premolars were decoronated, prepared to a standardized length of 15 mm, instrumented, and randomly divided into two groups (A and B) of 25 teeth each. Each group consisted of experimental (15 samples) and negative and positive controls (five samples each). The experimental groups were obturated with gutta-percha and root canal sealer. The positive control groups were obturated with a single cone of gutta-percha and root canal sealer. The outer surfaces (except for the apical 2 mm) were covered with two layers of nail varnish. An apparatus containing Brain Heart Infusion broth was designed, in which the teeth were placed. The samples in Group A were placed upside down, while Group B was placed normally. The coronal portions of the samples were placed in contact with fresh saliva. The number of days required for bacteria to penetrate the entire length of canals was determined. The samples were then immersed in India ink to determine the dye penetration configuration. Data were analyzed using Student's t-test.
The extent of dye penetration was significantly greater in Group B compared to Group A, and they were in a pattern rather than linear form.
Gravity and capillarity insignificantly affected bacterial leakage. Although gravity and capillarity did not affect bacterial penetration when applied to the coronal access of endodontically treated teeth, it seems that they can promote penetration of India ink into the canal after the bacterial test on the same tooth.
本研究旨在评估重力和毛细作用对人唾液细菌渗入根管充填全长的影响，并展示染料渗透形态。
选取50颗单根前磨牙，截冠后预备至标准化长度15毫米，进行根管预备，随机分为两组（A组和B组），每组25颗牙。每组包括实验组（15个样本）以及阴性和阳性对照组（各5个样本）。实验组用牙胶尖和根管封闭剂充填。阳性对照组用单根牙胶尖和根管封闭剂充填。牙齿外表面（根尖2毫米除外）涂两层指甲油。设计一个装有脑心浸液肉汤的装置，将牙齿放入其中。A组样本倒置放置，而B组正常放置。样本的冠部与新鲜唾液接触。确定细菌渗入根管全长所需的天数。然后将样本浸入印度墨水中以确定染料渗透形态。数据采用学生t检验进行分析。
与A组相比，B组的染料渗透程度显著更高，且呈一种形态而非线性形式。
重力和毛细作用对细菌渗漏影响不显著。虽然重力和毛细作用应用于根管治疗牙齿的冠部入口时不影响细菌渗入，但在同一颗牙齿进行细菌测试后，它们似乎能促进印度墨水渗入根管。
