Facultad de Medicina, Instituto de Investigaciones Clínicas "Dr. Américo Negrette", Universidad del Zulia, Maracaibo, Venezuela.
Influenza Other Respir Viruses. 2014 Jan;8(1):116-22. doi: 10.1111/irv.12155. Epub 2013 Aug 21.
Respiratory viral infections can induce different cytokine/chemokine profiles in lung tissues and have a significant influence on patients with asthma. There is little information about the systemic cytokine status in viral respiratory-infected asthmatic patients compared with non-asthmatic patients.
The aim of this study was to determine changes in circulating cytokines (IL-1β, TNF-α, IL-4, IL-5) and chemokines (MCP1: monocyte chemoattractant protein-1 and RANTES: regulated on activation normal T cell expressed and secreted) in patients with an asthmatic versus a non-asthmatic background with respiratory syncytial virus, parainfluenza virus or adenovirus respiratory infection. In addition, human monocyte cultures were incubated with respiratory viruses to determine the cytokine/chemokine profiles.
PATIENTS/METHODS: Patients with asthmatic (n = 34) and non-asthmatic (n = 18) history and respiratory infections with respiratory syncytial virus, parainfluenza, and adenovirus were studied. Healthy individuals with similar age and sex (n = 10) were used as controls. Cytokine/chemokine content in blood and culture supernatants was determined by ELISA. Monocytes were isolated by Hystopaque gradient and cocultured with each of the above-mentioned viruses.
Similar increased cytokine concentrations were observed in asthmatic and non-asthmatic patients. However, higher concentrations of chemokines were observed in asthmatic patients. Virus-infected monocyte cultures showed similar cytokine/chemokine profiles to those observed in the patients.
Circulating cytokine profiles induced by acute viral lung infection were not related to asthmatic status, except for chemokines that were already increased in the asthmatic status. Monocytes could play an important role in the increased circulating concentration of cytokines found during respiratory viral infections.
呼吸道病毒感染可在肺部组织中诱导不同的细胞因子/趋化因子谱，并对哮喘患者产生重大影响。与非哮喘患者相比，关于病毒呼吸道感染的哮喘患者的全身细胞因子状态的信息很少。
本研究旨在确定与非哮喘患者相比，呼吸道合胞病毒、副流感病毒或腺病毒呼吸道感染的哮喘和非哮喘患者的循环细胞因子（IL-1β、TNF-α、IL-4、IL-5）和趋化因子（MCP1：单核细胞趋化蛋白-1 和 RANTES：调节激活正常 T 细胞表达和分泌）的变化。此外，还用人单核细胞培养物孵育呼吸道病毒，以确定细胞因子/趋化因子谱。
患者/方法：研究了具有哮喘（n=34）和非哮喘（n=18）病史以及呼吸道合胞病毒、副流感病毒和腺病毒呼吸道感染的患者。具有相似年龄和性别（n=10）的健康个体被用作对照。通过 ELISA 测定血液和培养上清液中的细胞因子/趋化因子含量。通过 Hystopaque 梯度分离单核细胞，并与上述每种病毒共培养。
哮喘和非哮喘患者均观察到相似的细胞因子浓度升高。然而，哮喘患者观察到更高浓度的趋化因子。病毒感染的单核细胞培养物显示出与患者观察到的相似的细胞因子/趋化因子谱。
急性病毒性肺感染引起的循环细胞因子谱与哮喘状态无关，但在哮喘状态下已经增加的趋化因子除外。单核细胞在呼吸道病毒感染期间发现的循环细胞因子浓度增加中可能发挥重要作用。
