Prajuabjinda Onmanee, Panthong Sumalee, Itharat Arunporn
Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
J Med Assoc Thai. 2012 Jan;95 Suppl 1:S159-65.
Thai medicinal preparation of Khampramong Temple has been used for cancer treatment more than ten years ago. It is composed of eleven herbs. Many anticancer drugs exhibited antimicrobial activity as antitumor antibiotics such as the anthracycline group [daunorubicin] and quinone group [mitomycin C].
To determine antimicrobial activity of Thai medicinal plants used to treat cancer patients of Khampramong Temple by disc diffusion and agar dilution methods.
The extraction procedure was maceration method using 95% ethanol and drying by evaporator In the preliminary study, all extracts were evaluated for antimicrobial activity by disc diffusion method against two strains of Gram positive bacteria (Staphylococus aureus and Bacillus subtilis), one Gram negative bacteria (Escherichia coli) and one fungus (Candida albicans). The active plant extracts were diluted to determine the minimum inhibitory concentration (MIC) by agar dilution method.
The preparation showed antimicrobial activity against S. aureus, B. subtilis and E. coli (MIC = 1.25, 0.625 and 5 mg/ml, respectively) but no inhibition against Candida albicans. Most extracts showed activity against B. subtilis and Rhinacanthus nasutus extract showed the highest antimicrobial activity (MIC = 0.156 mg/ml). Hydnophytum formicarum Jack, Tectona grandis L.f and Salacia chinensis L. exhibited good antibacterial activity against S. aureus (MIC = 1.25, 0.15625 and 0.3125 mg/ml respectively).
These results supported the use of this preparation on chronic wound infection of cancer patients and the antimicrobial compounds of the preparation should be further studied to be used in cancer patients.
坎普拉蒙寺的泰国药用制剂在十多年前就已用于癌症治疗。它由十一种草药组成。许多抗癌药物都表现出抗菌活性，如蒽环类抗生素（柔红霉素）和醌类抗生素（丝裂霉素C）等抗肿瘤抗生素。
通过纸片扩散法和琼脂稀释法测定用于治疗坎普拉蒙寺癌症患者的泰国药用植物的抗菌活性。
提取方法为浸渍法，使用95%乙醇，并通过蒸发器干燥。在初步研究中，所有提取物均通过纸片扩散法对两种革兰氏阳性菌（金黄色葡萄球菌和枯草芽孢杆菌）、一种革兰氏阴性菌（大肠杆菌）和一种真菌（白色念珠菌）进行抗菌活性评估。对活性植物提取物进行稀释，通过琼脂稀释法测定最低抑菌浓度（MIC）。
该制剂对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌具有抗菌活性（MIC分别为1.25、0.625和5mg/ml），但对白色念珠菌无抑制作用。大多数提取物对枯草芽孢杆菌有活性，长穗猫尾草提取物的抗菌活性最高（MIC = 0.156mg/ml）。蚁栖植物、柚木和中华青牛胆对金黄色葡萄球菌表现出良好的抗菌活性（MIC分别为1.25、0.15625和0.3125mg/ml）。
这些结果支持将该制剂用于癌症患者的慢性伤口感染，该制剂的抗菌化合物应进一步研究，以便用于癌症患者。
