一种名为Kabpi的泰国传统药物提取物对口腔和咽喉感染的抗菌活性及其植物成分。

Antimicrobial activity of extracts from a Thai traditional remedy called Kabpi for oral and throat infection and its plant components.

作者信息

Lairungruang Kriyapa, Itharat Arunporn, Panthong Sumalee

出版信息

J Med Assoc Thai. 2014 Aug;97 Suppl 8:S108-15.

Abstract

BACKGROUND

Thai traditional remedy called Kabpi (KP) has long been usedfor oral and throat infection. It composed with four herbs Syzygium aromaticum (L.) Merr etPerry, Nigella sativa L., Limonia acidissima L. andAchasma sphaerocephalum Holtt.

OBJECTIVE

To investigate antimicrobial activity ofKP remedy extracts and its plant components.

MATERIAL AND METHOD

The extract procedures were maceration method with 95% ethanol, 50% ethanol and dried by evaporator and boiling in water, filtrated dried by lyophilizer The residue from the maceration in 95% ethanol and 50% ethanol were boiled in water and dried by lyophilizer In the preliminary studied, all extracts were evaluated antimicrobial activity by disc diffusion method. All extracts were tested against one type of gram positive bacteria Staphylococcus aureus (ATCC 25923) and one type ofyeast Candida albicans (ATCC 90028). The active plant extracts were diluted to determine the minimum inhibitory concentration (MIC) values by microtiter plate-based assay.

RESULTS

The 95% ethanol extract of KP remedy showed antimicrobial activity against S. aureus (ATCC 25923) and C. albicans (ATCC 90028) (MIC = 0.625, 0.625 mg/ml, respectively). The zone of inhibition ofall extracts were in the range of 7.33 to 23.33 mm. The 95% ethanol extract ofAchasma sphaerocephalum Holtt. showed the highest inhibition zone against S. aureus 21.00 mm (MIC = 0.625 mg/ml). The 95% ethanol extract ofSyzygium aromaticum (L.) Merr etPerry exhibited the best antimicrobial activity against C. albicans the inhibition zone with inhibition zone of23.33 mm (MIC = 0.156 mg/ml).

CONCLUSION

The present study demonstrated that the extracts ofKP remedy and its plant components had an antimicrobial effect against oral and throat infection such as S. aureus and C. albicans. These results support using this remedy of Thai traditional medicine called Kabpifor oral and throat infection.

摘要

背景

泰国传统药物Kabpi（KP）长期以来一直用于治疗口腔和咽喉感染。它由四种草药组成，分别是丁香（Syzygium aromaticum (L.) Merr et Perry）、黑种草（Nigella sativa L.）、酸橙（Limonia acidissima L.）和球头阿查斯马（Achasma sphaerocephalum Holtt）。

目的

研究KP药物提取物及其植物成分的抗菌活性。

材料与方法

提取方法采用浸渍法，分别用95%乙醇、50%乙醇进行浸渍，经蒸发干燥，再用水煮沸，经冷冻干燥过滤。95%乙醇和50%乙醇浸渍后的残渣用水煮沸，经冷冻干燥。在初步研究中，所有提取物均采用纸片扩散法评估抗菌活性。所有提取物均针对一种革兰氏阳性菌金黄色葡萄球菌（ATCC 25923）和一种酵母菌白色念珠菌（ATCC 90028）进行测试。通过微量滴定板法稀释活性植物提取物以确定最低抑菌浓度（MIC）值。

结果

KP药物的95%乙醇提取物对金黄色葡萄球菌（ATCC 25923）和白色念珠菌（ATCC 90028）具有抗菌活性（MIC分别为0.625、0.625 mg/ml）。所有提取物的抑菌圈范围为7.33至23.33 mm。球头阿查斯马的95%乙醇提取物对金黄色葡萄球菌的抑菌圈最大，为21.00 mm（MIC = 0.625 mg/ml）。丁香的95%乙醇提取物对白色念珠菌的抗菌活性最佳，抑菌圈为23.33 mm（MIC = 0.156 mg/ml）。