a Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, State Key Laboratory Breeding Base of Eco-Enviroment and Bio-Resource of the Three Gorges Area , School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University , Beibei, Chongqing , 400700 , China .
J Biomol Struct Dyn. 2014;32(10):1546-51. doi: 10.1080/07391102.2013.826143. Epub 2013 Aug 22.
Ribosome, the protein synthesis machinery essential for all living cells, consists of ribosomal proteins and RNA. Extraribosomal functions have recently been discovered for many ribosomal proteins, acting either as individual regulatory proteins or as a complex with other cell components. However, extraribosomal functions of Mycobacterium tuberculosis ribosomal proteins have not been systematically addressed. To this end, M. tuberculosis ribosomal proteins potentially engaged in extraribosomal functions were curated by data mining from transcriptional profiles of M. tuberculosis exposed to diverse treatments. Six M. tuberculosis ribosomal proteins, namely, S3 (Rv0707, rpsC), L16 (Rv0708, rplP), L29 (Rv0709, rpmC), S17 (Rv0710, rpsQ), S14 (Rv2056c, rpsN2), and L33 (Rv2057c, rpmG1), were found to behave idiosyncratically. The function of these abnormal ribosomal subunits can be further explored. Special emphasis is on their potential value as novel targets for better antibiotics.
核糖体是所有活细胞中必不可少的蛋白质合成机制，由核糖体蛋白和 RNA 组成。最近发现许多核糖体蛋白具有核糖体外的功能，它们可以作为单个调节蛋白发挥作用，也可以与其他细胞成分形成复合物发挥作用。然而，结核分枝杆菌核糖体蛋白的核糖体外功能尚未得到系统的研究。为此，我们通过对结核分枝杆菌在不同处理条件下的转录谱进行数据挖掘，对可能参与核糖体外功能的结核分枝杆菌核糖体蛋白进行了筛选。结果发现，结核分枝杆菌的 6 种核糖体蛋白（S3（Rv0707，rpsC）、L16（Rv0708，rplP）、L29（Rv0709，rpmC）、S17（Rv0710，rpsQ）、S14（Rv2056c，rpsN2）和 L33（Rv2057c，rpmG1））表现出异常。这些异常核糖体亚基的功能可以进一步探索。特别强调的是，它们作为新型靶点用于开发更好的抗生素的潜在价值。
