犬类狂犬病病毒免疫接种的细胞介导免疫反应研究。

Studies on cell-mediated immune response to rabies virus immunization in dogs.

作者信息

Jayakumar R, Ramadass P

机构信息

Department of Preventive Medicine, Madras Veterinary College, India.

出版信息

Vaccine. 1990 Aug;8(4):304-5. doi: 10.1016/0264-410x(90)90084-y.

DOI:10.1016/0264-410x(90)90084-y

PMID:2396468

Abstract

Peripheral blood leucocytes (PBL) from unvaccinated dogs and dogs vaccinated subcutaneously with beta-propiolactone (BPL) inactivated sheep brain antirabies vaccine were exposed in vitro to rabies antigen. A direct leucocyte migration inhibition (LMI) assay in agarose was used to study the cell-mediated immune (CMI) response. The CMI response could be detected as early as 7 days postvaccination. It reached a peak by 14 days postvaccination followed by a decline. The PBL from unvaccinated control dogs were not stimulated by rabies virus antigen.

摘要

将未接种疫苗的犬以及皮下接种β-丙内酯（BPL）灭活羊脑狂犬病疫苗的犬的外周血白细胞（PBL），在体外暴露于狂犬病抗原。采用琼脂糖直接白细胞迁移抑制（LMI）试验来研究细胞介导免疫（CMI）反应。CMI反应最早在接种疫苗后7天即可检测到。在接种疫苗后14天达到峰值，随后下降。未接种疫苗的对照犬的PBL未受到狂犬病病毒抗原的刺激。