Gerber J D, Sharpee R L, Swieczkowski T C, Beckenhauer W H
Vet Immunol Immunopathol. 1985 May;9(1):13-22. doi: 10.1016/0165-2427(85)90126-6.
Peripheral blood lymphocytes (PBL) from non-vaccinated dogs and from dogs either vaccinated intramuscularly (IM) or subcutaneously (SC) with an inactivated rabies virus vaccine (Rabguard-TC, Norden Laboratories, Lincoln, NE) or intramuscularly with an attenuated rabies virus vaccine (Endurall-R, Norden Laboratories, Lincoln, NE) were exposed in vitro to rabies virus. Blastogenesis of PBL was measured by incorporation of 3H-thymidine into the DNA of proliferating cells in the presence of a suboptimal concentration of phytohemagglutinin (PHA). Following the first vaccination, there was no difference in the blastogenic response of lymphocytes from dogs vaccinated IM with either the inactivated or attenuated rabies virus vaccines. The inactivated rabies vaccine stimulated as great or greater blastogenic response when it was given SC. The PBL from non-vaccinated control dogs were not stimulated by rabies virus. Dogs vaccinated with the inactivated vaccine developed a lymphocyte blastogenic response to rabies virus following challenge with virulent street rabies virus. Nonvaccinated control dogs did not develop a lymphocyte blastogenic response to rabies virus following challenge with virulent street rabies virus.
将未接种疫苗的犬以及分别通过肌肉注射（IM）或皮下注射（SC）接种灭活狂犬病病毒疫苗（Rabguard-TC，诺登实验室，林肯，内布拉斯加州）或通过肌肉注射接种减毒狂犬病病毒疫苗（Endurall-R，诺登实验室，林肯，内布拉斯加州）的犬的外周血淋巴细胞（PBL）在体外暴露于狂犬病病毒。通过在亚最佳浓度的植物血凝素（PHA）存在下将3H-胸腺嘧啶掺入增殖细胞的DNA中来测量PBL的增殖。首次接种疫苗后，用灭活或减毒狂犬病病毒疫苗进行肌肉注射的犬的淋巴细胞增殖反应没有差异。当皮下注射灭活狂犬病疫苗时，其刺激的增殖反应一样大或更大。未接种疫苗的对照犬的PBL未受到狂犬病病毒的刺激。用灭活疫苗接种的犬在用强毒街狂犬病病毒攻击后对狂犬病病毒产生淋巴细胞增殖反应。未接种疫苗的对照犬在用强毒街狂犬病病毒攻击后未对狂犬病病毒产生淋巴细胞增殖反应。
