Department of Medicine, Division of Gastroenterology, University of California, San Francisco, 513 Parnassus Avenue, Room S-357, San Francisco, CA 94143-0538, USA.
BMC Gastroenterol. 2013 Aug 22;13:131. doi: 10.1186/1471-230X-13-131.
The gut microbiome is altered in Crohn's disease. Although individual taxa have been correlated with post-operative clinical course, global trends in microbial diversity have not been described in this context.
We collected mucosal biopsies from the terminal ileum and ascending colon during surgery and post-operative colonoscopy in 6 Crohn's patients undergoing ileocolic resection (and 40 additional Crohn's and healthy control patients undergoing either surgery or colonoscopy). Using next-generation sequencing technology, we profiled the gut microbiota in order to identify changes associated with remission or recurrence of inflammation.
We performed 16S ribosomal profiling using 101 base-pair single-end sequencing on the Illumina GAIIx platform with deep coverage, at an average depth of 1.3 million high quality reads per sample. At the time of surgery, Crohn's patients who would remain in remission were more similar to controls and more species-rich than Crohn's patients with subsequent recurrence. Patients remaining in remission also exhibited greater stability of the microbiota through time.
These observations permitted an association of gut microbial profiles with probability of recurrence in this limited single-center study. These results suggest that profiling the gut microbiota may be useful in guiding treatment of Crohn's patients undergoing surgery.
肠道微生物群在克罗恩病中发生改变。虽然个别分类群与术后临床病程相关，但在这种情况下尚未描述微生物多样性的整体趋势。
我们在 6 名接受回肠结肠切除术的克罗恩病患者（以及另外 40 名接受手术或结肠镜检查的克罗恩病和健康对照患者）的手术和术后结肠镜检查期间收集末端回肠和升结肠的粘膜活检。我们使用下一代测序技术来分析肠道微生物群，以确定与炎症缓解或复发相关的变化。
我们使用 Illumina GAIIx 平台进行了 16S 核糖体基因测序，采用 101 个碱基的单端测序，深度覆盖，每个样本的平均深度为 130 万个高质量读数。在手术时，仍处于缓解期的克罗恩病患者与对照组更相似，且比随后复发的克罗恩病患者具有更多的物种。缓解期患者的微生物群也表现出更高的时间稳定性。
这些观察结果使我们能够在这项有限的单中心研究中，将肠道微生物群图谱与复发的可能性联系起来。这些结果表明，肠道微生物组的分析可能有助于指导接受手术的克罗恩病患者的治疗。
