Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
World J Gastroenterol. 2019 May 14;25(18):2204-2216. doi: 10.3748/wjg.v25.i18.2204.
The dysbiosis of the gut microbiome is evident in Crohn's disease (CD) compared with healthy controls (HC), although the alterations from active CD to remission after treatment are unclear.
To characterize the mucosa-associated gut microbiota in patients with CD before and after the induction therapy.
The basic information was collected from the subjects and the CD activity index (CDAI) was calculated in patients. A 16S rRNA sequencing approach was applied to determine the structures of microbial communities in mucosal samples including the terminal ileal, ascending colon, descending colon and rectum. The composition and function of mucosa-associated gut microbiota were compared between samples from the same cohort of patients before and after treatment. Differential taxa were identified to calculate the microbial dysbiosis index (MDI) and the correlation between MDI and CDAI was analyzed using Pearson correlation test. Predictive functional profiling of microbial communities was obtained with PICRUSt.
There were no significant differences in microbial richness among the four anatomical sites in individuals. Compared to active disease, the alpha diversity of CD in remission was increased towards the level of HC compared to the active stage. The principal coordinate analysis revealed that samples of active CD were clearly separated from those in remission, which clustered close to HC. Sixty-five genera were identified as differentially abundant between active and quiescent CD, with a loss of and a gain of potential beneficial bacteria including , , , and after the induction of remission. The combination of these taxa into a MDI showed a positive correlation with clinical disease severity and a negative correlation with species richness. The increased capacity for the inferred pathways including Lipopolysaccharide biosynthesis and Lipopolysaccharide biosynthesis proteins in patients before treatment negatively correlated with the abundance of , and .
The dysbiosis of mucosa-associated microbiota was associated with the disease phenotype and may become a potential diagnostic tool for the recurrence of disease.
与健康对照者（HC）相比，克罗恩病（CD）患者的肠道微生物组失调，尽管在治疗后从活动期 CD 到缓解期的变化尚不清楚。
描述 CD 患者在诱导治疗前后黏膜相关肠道微生物群。
收集受试者的基本信息，并计算 CD 患者的 CD 活动指数（CDAI）。应用 16S rRNA 测序方法确定黏膜样本中微生物群落的结构，包括末端回肠、升结肠、降结肠和直肠。比较同一队列患者治疗前后样本中黏膜相关肠道微生物群的组成和功能。鉴定差异分类群以计算微生物失调指数（MDI），并使用 Pearson 相关检验分析 MDI 与 CDAI 之间的相关性。使用 PICRUSt 获得微生物群落的预测功能分析。
个体中四个解剖部位的微生物丰富度没有显著差异。与活动期疾病相比，缓解期 CD 的 alpha 多样性向 HC 水平增加，与活动期相比。主坐标分析显示，活动期 CD 样本与缓解期样本明显分离，与 HC 聚类较近。在活动期和缓解期 CD 之间鉴定出 65 个差异丰度的属，在诱导缓解后，潜在有益细菌的损失和增加，包括 、 、 、 和 。将这些分类群组合成 MDI 显示与临床疾病严重程度呈正相关，与物种丰富度呈负相关。治疗前患者中推断途径的增加能力，包括内毒素生物合成和内毒素生物合成蛋白，与 、 和 的丰度呈负相关。
黏膜相关微生物群的失调与疾病表型相关，可能成为疾病复发的潜在诊断工具。
