在猪体内产生的抗犬瘟热病毒抗血清及其在麻疹病毒感染（犬瘟热、海豹瘟热、牛瘟）诊断程序中的应用。
Antiserum raised in pigs against canine distemper virus and its utility in diagnostic procedures for morbillivirus infections (canine distemper, phocine distemper, rinderpest).
作者信息
Zaghawa A, Liess B, Frey H R
机构信息
Institute of Virology, Hannover Veterinary School.
出版信息
Zentralbl Veterinarmed B. 1990 Jul;37(5):353-62. doi: 10.1111/j.1439-0450.1990.tb01069.x.
Antiserum against canine distemper virus (CDV) was raised in pigs by intranasal inoculation with CDV strains CND65 and ROCKBORN. Immunoglobulin fractions were conjugated with horseradish peroxidase. Peroxidase-conjugated anti-CDV immunoglobulin preparations were used for the detection and titration of CDV, seal-derived (phocine) distemper virus (PDV) and rinderpest virus (RPV) in Vero cell cultures. For the detection and titration of corresponding neutralizing antibodies a direct neutralizing peroxidase-linked antibody (NPLA) assay was established. The results were compared with those obtained with the conventional microtitre neutralization test (MNT) based on CPE reading. In addition the sensitivity of an indirect peroxidase-linked antibody (PLA) assay was tested in parallel with that of the NPLA assay using sera obtained from CDV-immunized pigs.
通过用犬瘟热病毒（CDV）毒株CND65和ROCKBORN经鼻内接种，在猪体内制备了抗犬瘟热病毒血清。将免疫球蛋白组分与辣根过氧化物酶结合。过氧化物酶结合的抗CDV免疫球蛋白制剂用于在Vero细胞培养物中检测和滴定CDV、海豹瘟热病毒（PDV）和牛瘟病毒（RPV）。为了检测和滴定相应的中和抗体，建立了直接中和过氧化物酶连接抗体（NPLA）试验。将结果与基于细胞病变效应（CPE）读数的传统微量滴定中和试验（MNT）所获得的结果进行比较。此外，使用从接种CDV的猪获得的血清，将间接过氧化物酶连接抗体（PLA）试验的灵敏度与NPLA试验的灵敏度进行了平行测试。
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