Alagendran S, Saibaba G, Muthukumar S, Rajkumar R, Guzman R G, Archunan G
Department of Animal Science, Centre for Pheromone Technology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Indian J Dent Res. 2013 Mar-Apr;24(2):157-63. doi: 10.4103/0970-9290.116669.
Predicting ovulation is the basis on which the fertile period is determined. Nowadays there are many methods available to detect the ovulatory period. Unfortunately, these methods are not always effective for accurate detection of ovulation. Hence, an attempt was made to detect ovulation through single dimension sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of protein with the help of saliva ferning.
The aim of this study was to determine the association of protein level with endogenous reproductive hormone level across the menstrual cycle.
Salivary protein and its confirmation were evaluated during menstrual cycle followed by SDS-PAGE and Mass spectrometry. STATISTICAL METHOD USED: The protein content present in saliva throughout menstrual cycle is trail by SPSS statistical software version.
Salivary proteins were investigated serially during pre-ovulatory, ovulatory and post-ovulatory periods of normal menstrual cycle in eighteen healthy volunteers. The samples were collected in three consecutive menstrual cycles. Salivary protein was estimated and analyzed by single dimension SDS-PAGE.
The results revealed significant variations in protein concentrations during the menstrual cycle. Protein levels were maximum during ovulation and minimum during postovulatory phase. Further, single dimension SDS-PAGE analysis showed seven different fractions of proteins is from 14-90 kilo Dalton (kDa) in the three phases of the menstrual cycle.
Among the proteins, 48 kDa protein was more predominantly exhibited during ovulatory phase than pre and post-ovulatory phase. The present study indicates that the protein level and the specific protein band (48 kDa) through MALDI-TOF MS analysis might serve as an indicator for ovulation.
预测排卵是确定排卵期的基础。如今有许多方法可用于检测排卵期。不幸的是，这些方法并非总能有效准确地检测排卵。因此，尝试借助唾液结晶通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）一维分析蛋白质来检测排卵。
本研究的目的是确定整个月经周期中蛋白质水平与内源性生殖激素水平之间的关联。
在月经周期中对唾液蛋白质及其验证进行评估，随后进行SDS-PAGE和质谱分析。所用统计方法：整个月经周期唾液中存在的蛋白质含量由SPSS统计软件版本进行追踪。
在18名健康志愿者的正常月经周期的排卵前、排卵期和排卵后阶段连续研究唾液蛋白质。样本在三个连续的月经周期中收集。唾液蛋白质通过一维SDS-PAGE进行估计和分析。
结果显示月经周期中蛋白质浓度存在显著变化。蛋白质水平在排卵时最高，在排卵后阶段最低。此外，一维SDS-PAGE分析显示在月经周期的三个阶段有7种不同的蛋白质组分，分子量从14 - 90千道尔顿（kDa）。
在这些蛋白质中，48 kDa蛋白质在排卵期比排卵前和排卵后阶段更显著地表现出来。本研究表明，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析的蛋白质水平和特定蛋白带（48 kDa）可能作为排卵的一个指标。
