Department of Chemistry and Biochemistry, National Chung Cheng University, No. 168, Sec. 1, University Rd., Min-Hsiung Township Chiayi, 621, Taiwan.
Phys Chem Chem Phys. 2013 Oct 21;15(39):16634-40. doi: 10.1039/c3cp51739j. Epub 2013 Aug 22.
Biological membranes are complex structures composed of various lipids and proteins. Different membrane compositions affect viscoelastic and hydrodynamic properties of membranes, which are critical to their functions. Lipid bilayer vesicles inserted by cholesterol not only enhance membrane surface motional behavior but also strengthen vesicle stability. Cholesterol-rich vesicles are similar to cell membranes in structure and composition. Therefore, cholesterol-rich vesicles can represent a typical model for studying membrane dynamics and functions. In this study, nuclear magnetic relaxation dispersion was used to investigate the detailed molecular dynamics of membrane differences between vesicles and cholesterol vesicles in the temperature range of 278-298 K. Vesicles of two different sizes were prepared. The effect of cholesterol mainly affected the order fluctuation of membranes and the diffusional motion of lipid molecules. In addition, phase variations were also observed in liposomes that contained cholesterol from analyses of the distances between lipid molecules.
生物膜是由各种脂质和蛋白质组成的复杂结构。不同的膜组成会影响膜的粘弹性和流体动力学性质，这对它们的功能至关重要。胆固醇插入的脂质双层囊泡不仅增强了膜表面的运动行为，而且增强了囊泡的稳定性。富含胆固醇的囊泡在结构和组成上与细胞膜相似。因此，富含胆固醇的囊泡可以作为研究膜动力学和功能的典型模型。在这项研究中，核磁共振弛豫色散用于研究温度范围为 278-298 K 时囊泡和胆固醇囊泡之间膜差异的详细分子动力学。制备了两种不同大小的囊泡。胆固醇的主要影响是膜的有序涨落和脂质分子的扩散运动。此外，还观察到含有胆固醇的脂质体在分子间距离的分析中出现了相变化。
