Wang Xue-Mei, Yan Mei, Liu Yu, Hailiqiguli Nu-Er-Mai-Mai-Ti
First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2013 Aug;15(8):633-7.
To detect the expression of surface molecule CD96 on stem cell (LSC) in children with acute leukemia, and to explore its clinical significance.
Bone marrow mononuclear cells were isolated in 69 children with newly diagnosed acute leukemia. CD34(+)CD38(-)CD123(+) LSCs were separated from these cells by flow cytometry (FCM) and then cultured, and CD96 expression on LSCs was detected by FCM. R-banding technique was used to analyze the karyotypes of the 69 children, and the data of their routine blood and immunological tests were collected.
CD96 was mainly expressed in children with acute myelogenous leukemia, and expressed to a lesser extent in those with acute lymphoblastic leukemia (P<0.05). The median expression level of CD96 in Uyghur children was 23.4%, versus 21.2% in Han children (P>0.05). The majority of children with CD96-positive children presented poor-prognosis karyotypes. Compared with CD96-negative children, children with CD96-positive children had a significantly lower complete remission rate (P<0.05) and significantly higher infection and relapse rates after chemotherapy (P<0.05).
Children with acute leukemia who have CD96-positive LSCs have a poor prognosis. CD96 may be a new indicator of prognosis in children with acute leukemia.
检测急性白血病患儿干细胞（白血病干细胞，LSC）表面分子CD96的表达，探讨其临床意义。
分离69例新诊断急性白血病患儿的骨髓单个核细胞，通过流式细胞术（FCM）从这些细胞中分离出CD34(+)CD38(-)CD123(+)白血病干细胞，然后进行培养，采用FCM检测白血病干细胞上CD96的表达。采用R显带技术分析69例患儿的核型，并收集其血常规及免疫检查数据。
CD96主要在急性髓系白血病患儿中表达，在急性淋巴细胞白血病患儿中表达程度较低（P<0.05）。维吾尔族患儿CD96表达水平中位数为23.4%，汉族患儿为21.2%（P>0.05）。多数CD96阳性患儿呈现预后不良核型。与CD96阴性患儿相比，CD96阳性患儿完全缓解率显著更低（P<0.05），化疗后感染及复发率显著更高（P<0.05）。
CD96阳性白血病干细胞的急性白血病患儿预后不良。CD96可能是急性白血病患儿预后的一个新指标。
