Ulitzer S
Arch Microbiol. 1975 Jun 20;104(1):67-71. doi: 10.1007/BF00447301.
Factors leading to swarming of Vibrio alginolyticus cells on solid media were studied. Polar flagellated rods from liquid medium develop into small colonies on solid medium. Byproducts, accumulating in the colony area, induce at certain critical concentrations, the formation of peritrichous flagella and development of long heavily flagellated filaments which swarm away form the high by-product concentrations. Several types of nonswarming mutants were isolated, among them, mutants which lack the capacity to form swarming-inducing pyproducts, but can be induced to swarm by byproducts of other mutants incapable of swarming. Different compounds could replace the natural metabolic byproducts; at very low concentration these compounds induce peritrichous flagella and swarming in some of the nonswarming mutants mentioned above. The natural metabolic byproducts accumulating in yeast-extract-peptone medium are suggested to be volatile acids belonging to the valine and isoleucine pathway. Wild-type V. alginolyticus cells cannot swarm on certain substrates but its mutants, able to swarm on many substrates in minimal media, are easily selected.
对导致溶藻弧菌细胞在固体培养基上群游的因素进行了研究。来自液体培养基的极生鞭毛杆菌在固体培养基上形成小菌落。在菌落区域积累的副产物，在达到一定临界浓度时，会诱导周生鞭毛的形成以及长的、鞭毛密集的丝状体的发育，这些丝状体从高副产物浓度区域群游离开。分离出了几种非群游突变体，其中包括缺乏形成群游诱导副产物能力但可被其他不能群游的突变体的副产物诱导群游的突变体。不同的化合物可以替代天然代谢副产物；在极低浓度下，这些化合物会诱导上述一些非群游突变体产生周生鞭毛并群游。酵母提取物 - 蛋白胨培养基中积累的天然代谢副产物被认为是缬氨酸和异亮氨酸途径中的挥发性酸。野生型溶藻弧菌细胞不能在某些底物上群游，但很容易筛选出能在基本培养基中的许多底物上群游的突变体。
