Liu Bing, Gao Qian, Zhang Xin, Chen Huizhen, Zhang Ying, Sun Yuehong, Yang Shan, Chen Chang
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 101408, China.
Microorganisms. 2021 Nov 18;9(11):2383. doi: 10.3390/microorganisms9112383.
, like other species, is a widely distributed marine bacterium that is able to outcompete other species in variable niches where diverse organic matters are supplied. However, it remains unclear how these cells sense and adjust metabolic flux in response to the changing environment. CsrA is a conserved RNA-binding protein that modulates critical cellular processes such as growth ability, central metabolism, virulence, and the stress response in -proteobacteria. Here, we first characterize the homolog in . The results show that CsrA activates swarming but not swimming motility, possibly by enhancing the expression of lateral flagellar associated genes. It is also revealed that CsrA modulates the carbon and nitrogen metabolism of , as evidenced by a change in the growth kinetics of various carbon and nitrogen sources when CsrA is altered. Quantitative RT-PCR shows that the transcripts of the genes encoding key enzymes involved in the TCA cycle and amino acid metabolism change significantly, which is probably due to the variation in mRNA stability given by CsrA binding. This may suggest that CsrA plays an important role in sensing and responding to environmental changes.
与其他物种一样，[具体物种名称未给出]是一种广泛分布的海洋细菌，能够在提供各种有机物的不同生态位中胜过其他物种。然而，目前尚不清楚这些细胞如何感知并响应不断变化的环境来调节代谢通量。CsrA是一种保守的RNA结合蛋白，可调节关键的细胞过程，如生长能力、中心代谢、毒力以及γ-变形菌中的应激反应。在此，我们首先对[具体物种名称未给出]中的CsrA同源物进行了表征。结果表明，CsrA激活群体运动但不激活游泳运动，可能是通过增强侧生鞭毛相关基因的表达来实现的。研究还表明，CsrA调节[具体物种名称未给出]的碳和氮代谢，当CsrA发生改变时，各种碳源和氮源的生长动力学变化证明了这一点。定量RT-PCR显示，参与三羧酸循环和氨基酸代谢的关键酶编码基因的转录本发生了显著变化，这可能是由于CsrA结合导致mRNA稳定性改变所致。这可能表明CsrA在感知和响应环境变化中发挥着重要作用。
