Division of Experimental Medicine, University of California San Francisco, San Francisco, California, USA.
J Virol. 2013 Nov;87(21):11924-9. doi: 10.1128/JVI.02128-13. Epub 2013 Aug 21.
Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We measured the gene expression of 34 antiviral factors in healthy peripheral blood mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family members. Expression of RTF1, RNA polymerase II-associated factor 1 (PAF1), TRIM11, TRIM26, and BST-2/tetherin correlated with decreased HIV-1 infectivity. This report demonstrates synchronous effects of activation-induced antiviral genes on HIV-1 infectivity, providing candidates for pharmacological manipulation.
细胞内在抗病毒因子的表达抑制 HIV-1 的复制。我们假设细胞的激活会调节宿主对 HIV-1 感染的限制和易感性。我们测量了健康外周血单核细胞 (PBMC) 中 34 种抗病毒因子的基因表达。细胞激活诱导了干扰素刺激基因 15 (ISG15)、三肽基 5α (TRIM5α)、骨髓基质细胞抗原 2 (BST-2)/ tetherin 和某些载脂蛋白 B mRNA 编辑酶 3 (APOBEC3) 家族成员的表达。RTF1、RNA 聚合酶 II 相关因子 1 (PAF1)、TRIM11、TRIM26 和 BST-2/tetherin 的表达与 HIV-1 感染性降低相关。本报告证明了激活诱导的抗病毒基因对 HIV-1 感染性的同步影响，为药理学干预提供了候选基因。
