细胞激活对原代细胞中抗 HIV-1 限制因子表达谱的影响。
Effects of cellular activation on anti-HIV-1 restriction factor expression profile in primary cells.
机构信息
Division of Experimental Medicine, University of California San Francisco, San Francisco, California, USA.
出版信息
J Virol. 2013 Nov;87(21):11924-9. doi: 10.1128/JVI.02128-13. Epub 2013 Aug 21.
Abstract
Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We measured the gene expression of 34 antiviral factors in healthy peripheral blood mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family members. Expression of RTF1, RNA polymerase II-associated factor 1 (PAF1), TRIM11, TRIM26, and BST-2/tetherin correlated with decreased HIV-1 infectivity. This report demonstrates synchronous effects of activation-induced antiviral genes on HIV-1 infectivity, providing candidates for pharmacological manipulation.
摘要
细胞内在抗病毒因子的表达抑制 HIV-1 的复制。我们假设细胞的激活会调节宿主对 HIV-1 感染的限制和易感性。我们测量了健康外周血单核细胞 (PBMC) 中 34 种抗病毒因子的基因表达。细胞激活诱导了干扰素刺激基因 15 (ISG15)、三肽基 5α (TRIM5α)、骨髓基质细胞抗原 2 (BST-2)/ tetherin 和某些载脂蛋白 B mRNA 编辑酶 3 (APOBEC3) 家族成员的表达。RTF1、RNA 聚合酶 II 相关因子 1 (PAF1)、TRIM11、TRIM26 和 BST-2/tetherin 的表达与 HIV-1 感染性降低相关。本报告证明了激活诱导的抗病毒基因对 HIV-1 感染性的同步影响，为药理学干预提供了候选基因。
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本文引用的文献
1
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Retrovirology. 2012 Oct 23;9:87. doi: 10.1186/1742-4690-9-87.
2
The restriction factors of human immunodeficiency virus.人类免疫缺陷病毒的限制因素。
J Biol Chem. 2012 Nov 30;287(49):40875-83. doi: 10.1074/jbc.R112.416925. Epub 2012 Oct 5.
3
Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11.基于密码子使用的人 Schlafen 11 抑制 HIV 蛋白合成。
Nature. 2012 Nov 1;491(7422):125-8. doi: 10.1038/nature11433. Epub 2012 Sep 23.
4
SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.SAMHD1 限制静息 CD4(+) T 细胞中的 HIV-1 感染。
Nat Med. 2012 Nov;18(11):1682-7. doi: 10.1038/nm.2964.
5
HIV-1 infection of human macrophages directly induces viperin which inhibits viral production.HIV-1 感染人巨噬细胞可直接诱导 viperin 的产生，而 viperin 可抑制病毒的产生。
Blood. 2012 Jul 26;120(4):778-88. doi: 10.1182/blood-2012-01-407395. Epub 2012 Jun 7.
6
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Virology. 2012 Aug 15;430(1):1-9. doi: 10.1016/j.virol.2012.03.018. Epub 2012 May 12.
7
Role of retroviral restriction factors in the interferon-α-mediated suppression of HIV-1 in vivo.逆转录病毒限制因子在干扰素-α体内抑制 HIV-1 中的作用。
Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3035-40. doi: 10.1073/pnas.1111573109. Epub 2012 Feb 6.
8
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Retrovirology. 2011 Nov 14;8:94. doi: 10.1186/1742-4690-8-94.
10
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PLoS Pathog. 2011 Sep;7(9):e1002221. doi: 10.1371/journal.ppat.1002221. Epub 2011 Sep 22.
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