水痘-带状疱疹病毒开放阅读框 48 编码一种有活性的核酸酶。
Varicella-zoster virus open reading frame 48 encodes an active nuclease.
机构信息
Departments of Ophthalmology.
出版信息
J Virol. 2013 Nov;87(21):11936-8. doi: 10.1128/JVI.01879-13. Epub 2013 Aug 21.
Abstract
Based on a DNA sequence and relative genomic position similar to those other herpesviruses, varicella-zoster virus (VZV) open reading frame 48 (ORF48) is predicted to encode an alkaline nuclease. Here we report the cloning, expression, purification, and characterization of recombinant VZV ORF48 protein and a VZV ORF48 point mutation (T172P). Protein encoded by wild-type ORF48, but not mutant protein, displayed both endo- and exonuclease activity, identifying ORF48 as a potential therapeutic target in VZV disease since efficient viral replication requires viral nuclease activity.
摘要
根据 DNA 序列和相对基因组位置与其他疱疹病毒相似，水痘带状疱疹病毒（VZV）开放阅读框 48（ORF48）预计编码碱性核酸酶。在这里，我们报告了重组 VZV ORF48 蛋白和 VZV ORF48 点突变（T172P）的克隆、表达、纯化和特性。野生型 ORF48 编码的蛋白，但突变蛋白没有，显示了内切核酸酶和外切核酸酶活性，这表明 ORF48 是 VZV 疾病的潜在治疗靶点，因为有效的病毒复制需要病毒核酸酶活性。
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