rpoB 基因 PCR 检测方法对 whipplei 螺旋体的验证：国家参考实验室 10 年的经验。
Validation of an rpoB gene PCR assay for detection of Tropheryma whipplei: 10 years' experience in a National Reference Laboratory.
机构信息
Institut für Mikrobiologie und Hygiene, Charité-Universitätsmedizin Berlin, Berlin, Germany.
出版信息
J Clin Microbiol. 2013 Nov;51(11):3858-61. doi: 10.1128/JCM.01703-13. Epub 2013 Aug 21.
Abstract
The performance of a real-time PCR assay targeting the Tropheryma whipplei rpoB gene was evaluated using test strains and 1,236 clinical specimens in a national reference laboratory. The novel rpoB-PCR assay proved to be specific, revealed improved analytical sensitivity, and substantially accelerated detection of T. whipplei DNA in clinical specimens.
摘要
采用靶向 whipple 螺旋体 rpoB 基因的实时 PCR 检测方法，在国家级参考实验室使用检测菌株和 1236 例临床标本对该方法进行了评估。新型 rpoB-PCR 检测方法特异性强，分析灵敏度显著提高，大大加快了临床标本中 whipple 螺旋体 DNA 的检测速度。
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