通过单分子光谱检测到的动力学亚群：功能性核酸的基本特性还是实验假象？

Kinetic subpopulations detected by single-molecule spectroscopy: fundamental property of functional nucleic acids or experimental artefact?

作者信息

König Sebastian L B, Kowerko Danny, Sigel Roland K O

机构信息

Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zürich.

出版信息

Chimia (Aarau). 2013;67(4):240-3. doi: 10.2533/chimia.2013.240.

DOI:10.2533/chimia.2013.240

PMID:23967697

Abstract

Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1* interaction, two RNA sequences that play an important role in group II intron mediated self-cleavage. Further examples of dynamic heterogeneity in functional nucleic acids are provided and the possible origins of this phenomenon are discussed.

摘要

单分子光谱技术能够直接观察单个生物分子的构象动力学。在此，我们描述了单分子荧光共振能量转移（smFRET）如何揭示EBS1*/IBS1*相互作用中的异质动力学，这两个RNA序列在II组内含子介导的自我切割中发挥着重要作用。文中还提供了功能性核酸中动态异质性的更多实例，并讨论了这一现象可能的起源。