The Institute for Molecular Engineering, The University of Chicago, Chicago, IL 60637, USA.
Nanoscale. 2016 Dec 8;8(48):19928-19944. doi: 10.1039/c6nr06794h.
Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying the conformation dynamics and interactions of individual biomolecules. In this review, we describe the concept and principle of smFRET, illustrate general instrumentation and microscopy settings for experiments, and discuss the methods and algorithms for data analysis. Subsequently, we review applications of smFRET in protein conformational changes, ion channel open-close properties, receptor-ligand interactions, nucleic acid structure regulation, vesicle fusion, and force induced conformational dynamics. Finally, we discuss the main limitations of smFRET in molecular biology.
单分子荧光共振能量转移（smFRET）是研究单个生物分子构象动力学和相互作用的有力技术。在这篇综述中，我们描述了 smFRET 的概念和原理，说明了实验的一般仪器和显微镜设置，并讨论了数据分析的方法和算法。随后，我们回顾了 smFRET 在蛋白质构象变化、离子通道开闭特性、受体-配体相互作用、核酸结构调控、囊泡融合以及力诱导构象动力学中的应用。最后，我们讨论了 smFRET 在分子生物学中的主要局限性。
