Laboratório de Biologia Sintética, EMBRAPA Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Av. W5 N, Brasília, DF, 70.770-900, Brazil.
Curr Protein Pept Sci. 2013 Nov;14(7):568-79. doi: 10.2174/13892037113149990072.
Recombinant DNA technology has allowed the ectopic production of proteins and peptides of different organisms leading to biopharmaceutical production in large cultures of bacterial, yeasts and mammalian cells. Otherwise, the expression of recombinant proteins and peptides in plants is an attractive alternative presenting several advantages over the commonly used expression systems including reduced production costs, easy scale-up and reduced risks of pathogen contamination. Different types of proteins and peptides have been expressed in plants, including antibodies, antigens, and proteins and peptides of medical, veterinary and industrial applications. However, apart from providing a proof of concept, the use of plants as platforms for heterologous protein and peptide production still depends on key steps towards optimization including the enhancement of expression levels, manipulation of post-transcriptional modifications and improvements in purification methods. In this review, strategies to increase heterologous protein and peptide stability and accumulation are discussed, focusing on the expression of peptides through the use of gene fusions.
重组 DNA 技术允许不同生物体的蛋白质和肽的异位生产，从而导致细菌、酵母和哺乳动物细胞的大规模培养中的生物制药生产。否则，重组蛋白和肽在植物中的表达是一种有吸引力的替代方法，与常用的表达系统相比具有几个优势，包括降低生产成本、易于扩大规模和降低病原体污染的风险。不同类型的蛋白质和肽已在植物中表达，包括抗体、抗原以及具有医学、兽医和工业应用的蛋白质和肽。然而，除了提供概念验证外，植物作为异源蛋白质和肽生产平台的使用仍然取决于优化的关键步骤，包括提高表达水平、操纵转录后修饰和改进纯化方法。在本文综述中，讨论了提高异源蛋白质和肽稳定性和积累的策略，重点是通过使用基因融合来表达肽。
