Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
Biosens Bioelectron. 2014 Jan 15;51:232-7. doi: 10.1016/j.bios.2013.07.039. Epub 2013 Aug 3.
In this work, a novel label-free chemiluminescent (CL) aptasensor has been developed for rapid and facile detection of adenosine triphosphate (ATP, as model analyte) using graphene oxide (GO) nano-platform. The strategy relies on the preferential binding of GO to single-stranded DNA (ssDNA) over rigid double-stranded DNA (dsDNA) or aptamer-target complexes, and the instantaneous derivative reaction between phenylglyoxal (PGO), a special CL reagent as the signaling molecule, and guanine nucleobases (G) of aptamer strands adsorbed on the surface of GO. In the absence of ATP, the aptamers adsorbed onto the surface of GO leading to a strong background CL signal. Conversely, in the presence of ATP, the aptamers formed the aptamer-ATP complexes which had weak binding ability to GO resulting in a significant CL signal decrease. The CL intensity was adversely related to the ATP concentration in the assay solution. The biosensor's signal decreased linearly with the logarithm of the concentration of ATP from 2 to 80 nmol with a detection limit of 1.4 nmol. The aptasensor also showed high selectivity against cytosine triphosphate (CTP), guanosine triphosphate (GTP), and uridine triphosphate (UTP). The method presented here holds the advantages of being label-free, cost effective, rapid, sensitive and selective, which would shows great promise for clinical application.
在这项工作中，开发了一种新型的无标记化学发光（CL）适体传感器，用于使用氧化石墨烯（GO）纳米平台快速简便地检测三磷酸腺苷（ATP，作为模型分析物）。该策略依赖于 GO 对单链 DNA（ssDNA）的优先结合，而不是刚性双链 DNA（dsDNA）或适体 - 靶复合物，以及苯乙二醛（PGO）与吸附在 GO 表面的适体链上的鸟嘌呤核苷碱基（G）之间的瞬时衍生反应，PGO 是一种特殊的 CL 试剂作为信号分子。在没有 ATP 的情况下，吸附在 GO 表面上的适体导致强烈的背景 CL 信号。相反，在存在 ATP 的情况下，适体形成与 GO 具有弱结合能力的适体-ATP 复合物，导致 CL 信号显著降低。CL 强度与测定溶液中 ATP 的浓度呈负相关。该生物传感器的信号随 ATP 浓度的对数从 2 到 80 nmol 线性降低，检测限为 1.4 nmol。该适体传感器对胞嘧啶三磷酸（CTP）、鸟嘌呤三磷酸（GTP）和尿嘧啶三磷酸（UTP）也表现出高选择性。这里提出的方法具有无标记、经济高效、快速、灵敏和选择性的优点，这将为临床应用带来巨大的希望。
