State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Sensors (Basel). 2018 Sep 29;18(10):3281. doi: 10.3390/s18103281.
In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R² = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 10⁶ to 2.988 × 10⁶ M. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive.
在这项研究中，开发了一种无标记荧光、无酶、简单、高灵敏度和逻辑门适体传感器，用于检测三磷酸腺苷 (ATP)。带有粘性末端的双链脱氧核糖核酸 (DNA) 被连接到氧化石墨烯 (GO) 上，形成适体传感器探针。ATP 和单链 DNA 被用作输入信号。PicoGreen 染料的荧光强度被用作输出信号。研究了与生物传感器相关的性能，包括逻辑门结构、反应时间、线性度、灵敏度和特异性，结果表明成功构建了一个与门。发现 ATP 的检测范围为 20 到 400 nM（R²=0.9943），检测限 (LOD) 为 142.6 pM，灵敏度范围为 1.846×10⁶ 到 2.988×10⁶ M。这种检测 ATP 的方法具有简单、低成本和高灵敏度的特点。
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