功能化石墨烯作为敏感电化学标签，用于基于目标的分裂适体传感器的连接，用于双重检测。
Functionalized graphene as sensitive electrochemical label in target-dependent linkage of split aptasensor for dual detection.
机构信息
Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Chinese Academy of Sciences, Beijing 100039, China.
Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
出版信息
Biosens Bioelectron. 2014 Dec 15;62:52-8. doi: 10.1016/j.bios.2014.06.008. Epub 2014 Jun 11.
A new type of electrochemical aptasensor was reported here for highly sensitive detection of adenosine triphosphate (ATP) and adenosine deaminase (ADA) activity using functionalized graphene as efficient electrochemical label. The specific binding of ATP and its aptamer could link the split aptamers modified graphene and magnetic beads together. After ADA catalysis and magnetic separation, graphene material anchored on electrode surface would efficiently facilitate electron transfer, thus produce detectable electrochemical signals. The detection limits for ATP and ADA activity were 13.6 nM and 0.01 unit/mL (~1.2 nM), respectively. Our work would supply new horizons for the diagnostic applications of graphene-based materials in biomedicine and biosensors.
本文报道了一种新型的电化学适体传感器，用于高灵敏度检测三磷酸腺苷（ATP）和腺苷脱氨酶（ADA）的活性，该传感器使用功能化石墨烯作为有效的电化学标记物。ATP 与其适体的特异性结合可以将修饰石墨烯的分裂适体连接在一起，并通过磁分离。ADA 催化和磁分离后，固定在电极表面的石墨烯材料可以有效地促进电子转移，从而产生可检测的电化学信号。ATP 和 ADA 活性的检测限分别为 13.6 nM 和 0.01 单位/mL（~1.2 nM）。我们的工作将为基于石墨烯的材料在生物医学和生物传感器中的诊断应用提供新的前景。
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