Division for Biochemistry of Joint and Connective Tissue Diseases, Department of Orthopedic Surgery, University of Ulm, Germany.
Biomaterials. 2013 Nov;34(35):8851-9. doi: 10.1016/j.biomaterials.2013.08.010. Epub 2013 Aug 19.
Hexagonally arranged Gold nanoparticles with controllable diameters and inter-particle distances were deposited on thick SiO2 layers on top of Si wafers and used as masks during subsequent reactive ion etching. In this way, arrays of nanopillars are obtained with well-defined diameters (10/30 nm), inter-pillar distances (50-120 nm) and heights (20-35 nm), all on the nanoscale. Such nanotopographies served as substrate for multipotent mesenchymal stromal cells (MSC) and human osteoblasts (OB) allowing to study cellular responses to purely topographically patterned interfaces. Focus was put on adhesion, proliferation and differentiation of the cells. It turned out experimentally that adhesion is comparable for both cell types practically independent of topographical details at the substrate surface. Topography induced proliferation enhancement, however, is again independent of geometrical details in case of MSC, but significantly sensitive to pillar height in case of OB with a clear preference towards short nanopillars (20 nm). A high sensitivity to topographic details is also observed for osteogenic differentiation of MSC, in that case with a preference towards higher nanopillars (50 nm). The present experimental data also allow the important conclusion that cell proliferation and differentiation can be optimized simultaneously by fine-tuning nanoscaled topographical parameters.
在硅片顶部的厚二氧化硅层上沉积具有可控直径和粒子间距离的六边形排列的金纳米粒子，并在随后的反应离子刻蚀中用作掩模。通过这种方式，可以获得具有明确定义的直径（10/30nm）、柱间距（50-120nm）和高度（20-35nm）的纳米柱阵列，所有这些都在纳米尺度上。这种纳米形貌作为多能间充质基质细胞（MSC）和人成骨细胞（OB）的基底，允许研究细胞对纯形貌图案化界面的反应。重点放在细胞的粘附、增殖和分化上。实验结果表明，两种细胞类型的粘附几乎相同，实际上与基底表面的形貌细节无关。然而，对于 MSC，增殖增强是由形貌诱导的，与几何细节无关，但对于 OB 而言，柱高则明显敏感，短纳米柱（20nm）具有明显的偏好。MSC 的成骨分化对形貌细节也非常敏感，在这种情况下，更喜欢更高的纳米柱（50nm）。目前的实验数据还允许得出一个重要结论，即通过微调纳米形貌参数，可以同时优化细胞增殖和分化。
