Wei Tao, Jin Xiao Xiao, Xu Tian Jun
Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, 316000 Zhoushan, People's Republic of China.
J Genet. 2013;92(2):247-57. doi: 10.1007/s12041-013-0259-6.
To understand the phylogenetic position of Bostrychus sinensis in Eleotridae and the phylogenetic relationships of the family, we determined the nucleotide sequence of the mitochondrial (mt) genome of Bostrychus sinensis. It is the first complete mitochondrial genome sequence of Bostrychus genus. The entire mtDNA sequence was 16508 bp in length with a standard set of 13 protein-coding genes, 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a noncoding control region. The mitochondrial genome of B. sinensis had common features with those of other bony fishes with respect to gene arrangement, base composition, and tRNA structures. Phylogenetic hypotheses within Eleotridae fish have been controversial at the genus level. We used the mitochondrial cytochrome b (cytb) gene sequence to examine phylogenetic relationships of Eleotridae by using partitioned Bayesian method. When the specific models and parameter estimates were presumed for partitioning the total data, the harmonic mean -lnL was improved. The phylogenetic analysis supported the monophyly of Hypseleotris and Gobiomorphs. In addition, the Bostrychus were most closely related to Ophiocara, and the Philypnodon is also the sister to Microphlypnus, based on the current datasets. Further, extensive taxonomic sampling and more molecular information are needed to confirm the phylogenetic relationships in Eleotridae.
为了解中华乌塘鳢在塘鳢科中的系统发育位置以及该科的系统发育关系，我们测定了中华乌塘鳢线粒体（mt）基因组的核苷酸序列。这是乌塘鳢属的首个完整线粒体基因组序列。整个mtDNA序列长度为16508 bp，包含一套标准的13个蛋白质编码基因、22个转运RNA基因（tRNAs）、两个核糖体RNA基因（rRNAs）和一个非编码控制区。中华乌塘鳢的线粒体基因组在基因排列、碱基组成和tRNA结构方面与其他硬骨鱼类具有共同特征。塘鳢科鱼类在属级水平的系统发育假说一直存在争议。我们使用线粒体细胞色素b（cytb）基因序列，采用分区贝叶斯方法来研究塘鳢科的系统发育关系。当假定用于划分总数据的特定模型和参数估计时，调和平均-lnL得到了改善。系统发育分析支持 Hypseleotris 和 Gobiomorphs 的单系性。此外，基于当前数据集，乌塘鳢与裸项栉鰕虎鱼关系最为密切，而菲律宾栉鰕虎鱼也是微栉鰕虎鱼的姐妹群。此外，需要广泛的分类采样和更多的分子信息来确认塘鳢科中的系统发育关系。
