Programa de Pós-Graduação em Biotecnologia, Universidade do Estado do Amazonas, Manaus, AM.
Rev Soc Bras Med Trop. 2013 Jul-Aug;46(4):411-9. doi: 10.1590/0037-8682-0063-2013.
In vitro bioassays were performed to access the larvicidal activity of crude extracts from the endophytic fungus Pestalotiopsis virgulata (Melanconiales, Amphisphaeriaceae) and the saprophytic fungus Pycnoporus sanguineus (Basidiomycetes, Polyporaceae) against the mosquitoes Aedes aegypti and Anopheles nuneztovari.
The extracts were tested at concentrations of 100, 200, 300, 400 and 500ppm. Ethyl acetate mycelia (EAM) extracts and liquid culture media (LCM) from Pe. virgulata and Py. sanguineus were tested against third instar larvae of Ae. aegypti and An. nuneztovari.
The larvicidal activity of the EAM extracts from Pe. virgulata against Ae. aegypti had an LC50=101.8ppm, and the extract from the basidiomycete fungus Py. sanguineus had an LC50=156.8ppm against the Ae. aegypti larvae. The Pe. virgulata extract had an LC50=16.3ppm against the An. nuneztovari larvae, and the Py. sanguineus extract had an LC50=87.2ppm against these larvae.
These results highlight the larvicidal effect of EAM extracts from the endophyte Pe. virgulata against the two larval mosquitoes tested. Thus, Pe. virgulata and Py. sanguineus have the potential for the production of bioactive substances against larvae of these two tropical disease vectors, with An. nuneztovari being more susceptible to these extracts.
进行了体外生物测定，以评估内生真菌拟盘多毛孢（Melanconiales，盘菌科）和腐生真菌红栓菌（担子菌纲，多孔菌科）粗提物对埃及伊蚊和疟蚊的幼虫的杀幼虫活性。
以 100、200、300、400 和 500ppm 的浓度测试提取物。测试了拟盘多毛孢和红栓菌的乙酸乙酯菌丝体（EAM）提取物和液体培养基（LCM）对第三龄埃及伊蚊和疟蚊幼虫的作用。
拟盘多毛孢 EAM 提取物对埃及伊蚊的杀幼虫活性的 LC50=101.8ppm，而担子菌真菌红栓菌的提取物对埃及伊蚊幼虫的 LC50=156.8ppm。拟盘多毛孢提取物对疟蚊幼虫的 LC50=16.3ppm，红栓菌提取物对疟蚊幼虫的 LC50=87.2ppm。
这些结果突出了内生真菌拟盘多毛孢 EAM 提取物对两种幼虫蚊子的杀幼虫作用。因此，拟盘多毛孢和红栓菌具有产生针对这两种热带病传播媒介幼虫的生物活性物质的潜力，其中疟蚊对这些提取物更为敏感。
