Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea.
Chem Soc Rev. 2014 Feb 21;43(4):1007-13. doi: 10.1039/c3cs60184f.
Since its first demonstration about twenty years ago, single-molecule fluorescence resonance energy transfer (FRET) has undergone remarkable technical advances. In this tutorial review, we will discuss two technical advances that increase the information content of the single-molecule FRET measurements: single-molecule multi-color FRET and single-molecule FRET combined with force or torque. Our expectations for future developments will be briefly discussed at the end.
自二十年前首次展示以来,单分子荧光共振能量转移(FRET)技术取得了显著的技术进步。在本教程综述中,我们将讨论提高单分子 FRET 测量信息量的两项技术进展:单分子多色 FRET 和单分子 FRET 与力或扭矩的结合。最后,我们将简要讨论对未来发展的期望。