Zhang Meng, Li Yinan, Xu Dalin, Wang Wenyue, Zhou Hong, Yang Xiaoyan
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Qingdao Jimo District Comprehensive Inspection and Testing Center, Qingdao, China.
Mikrochim Acta. 2025 Apr 26;192(5):323. doi: 10.1007/s00604-025-07172-3.
Vitamin D, mainly in the active form of 25-hydroxy vitamin D (25(OH)D), participates in blood circulation and plays a vital role in metabolic homeostasis. Herein, an electrochemiluminescent (ECL)/colorimetric dual-analytical biosensing platform based on HOFs-g-CN-CeO (HCC) composite was developed for 25(OH)D detection. In the ECL system, electrochemiluminescence-resonance energy transfer (ECL-RET) occurred utilizing HCC as the ECL donor and gold nanorods (Au NRs) as the acceptor. The binding of the complementary aptamer chain (ssDNA) labeled with Au NRs to the aptamer chain VDBA14 immobilized on the surface of HCC quenched the ECL emission via ECL-RET. After adding the target 25(OH)D, ECL response was recovered due to the competition for VDBA14 between ssDNA and 25(OH)D, resulting in the reduction of Au NRs and attenuation of the ECL-RET on the electrode. In the colorimetric system, VDBA14 immobilized on magnetic beads (MBs) captured ssDNA tagged with HCC through complementary base pairing. VDBA14 could specifically recognize 25(OH)D to release ssDNA competitively, and then, the released HCC was used as the nanozyme to catalyze the chromogenic reaction between tetramethylbenzidine (TMB) and HO. The bimodal analysis showed a good response to 25(OH)D in the linear range 20 ~ 80 ng/mL, with detection limits of 15.91 ng/mL (for the ECL system) and 7.28 ng/mL (for the colorimetric system). The proposed dual-analytical biosensor improved the reliability of detecting 25(OH)D and performed satisfactorily in practical applications, which is of great significance in immunomodulation, skin physiology, and other important aspects of human health.
维生素D主要以25-羟基维生素D（25(OH)D）的活性形式参与血液循环，并在代谢稳态中发挥至关重要的作用。在此，基于金属有机框架-石墨相氮化碳-二氧化铈（HOFs-g-CN-CeO，HCC）复合材料构建了一种用于检测25(OH)D的电化学发光（ECL）/比色双分析生物传感平台。在ECL体系中，以HCC作为ECL供体，金纳米棒（Au NRs）作为受体，发生了电化学发光共振能量转移（ECL-RET）。标记有Au NRs的互补适配体链（ssDNA）与固定在HCC表面的适配体链VDBA14结合，通过ECL-RET淬灭了ECL发射。加入目标物25(OH)D后，由于ssDNA与25(OH)D之间对VDBA14的竞争，ECL响应得以恢复，导致电极上Au NRs减少以及ECL-RET减弱。在比色体系中，固定在磁珠（MBs）上的VDBA14通过互补碱基配对捕获标记有HCC的ssDNA。VDBA14能够特异性识别25(OH)D以竞争性释放ssDNA，然后，释放出的HCC用作纳米酶催化四甲基联苯胺（TMB）与过氧化氢（HO）之间的显色反应。双峰分析表明，该平台在20～80 ng/mL的线性范围内对25(OH)D具有良好的响应，检测限分别为15.91 ng/mL（ECL体系）和7.28 ng/mL（比色体系）。所提出的双分析生物传感器提高了检测25(OH)D的可靠性，并在实际应用中表现良好，这在免疫调节、皮肤生理学以及人类健康的其他重要方面具有重要意义。
