Lee Dongkyu, Kim Dowon, Kim Youngwoong, Park Ki-Hyun, Oh Eun-Jee, Kim Yonggoo, Kim Byungkyu
1School of Aerospace and Mechanical Engineering, Nano Bio Robotics Lab, Korea Aerospace University, Korea.
J Lab Autom. 2014 Feb;19(1):60-74. doi: 10.1177/2211068213498385. Epub 2013 Aug 22.
We present a negative dielectrophoresis (n-DEP)-based cell separation system for high-throughput and high-efficiency cell separation. To achieve a high throughput, the proposed system comprises macro-sized channel and cantilever-type electrode (CE) arrays (L × W × H = 150 µm × 500 µm × 50 µm) to generate n-DEP force. For high efficiency, double separation modules, which have macro-sized channels and CE arrays in each separation module, are employed. In addition, flow regulators to precisely control the hydrodynamic force are allocated for each outlet. Because the hydrodynamic force and the n-DEP force acting on the target cell are the main determinants of the separation efficiency, we evaluate the theoretical amount of hydrodynamic force and n-DEP force acting on each target cell. Based on theoretical results, separation conditions are experimentally investigated. Finally, to demonstrate the separation performance, we performed the separation of target cells (live K562) from nontarget cells (dead K562) under conditions of low voltage (7Vp-p with 100 kHz) and a flow rate of 15 µL•min⁻¹, 6 µL•min⁻¹, and 8 µL•min⁻¹ in outlets 1, 2, and 3, respectively. The system can separate target cells with 95% separation efficiency in the case of the ratio of 5:1 (live K562:dead K562).
我们提出了一种基于负介电泳（n-DEP）的细胞分离系统，用于高通量、高效率的细胞分离。为实现高通量，该系统包括宏观尺寸的通道和悬臂式电极（CE）阵列（长×宽×高 = 150 µm×500 µm×50 µm）以产生n-DEP力。为提高效率，采用了双分离模块，每个分离模块都有宏观尺寸的通道和CE阵列。此外，为每个出口配置了用于精确控制流体动力的流量调节器。由于作用在目标细胞上的流体动力和n-DEP力是分离效率的主要决定因素，我们评估了作用在每个目标细胞上的流体动力和n-DEP力的理论值。基于理论结果，对分离条件进行了实验研究。最后，为证明分离性能，我们在低电压（7Vp-p，100 kHz）以及出口1、2、3的流速分别为15 µL•min⁻¹、6 µL•min⁻¹和8 µL•min⁻¹的条件下，进行了从非目标细胞（死K562）中分离目标细胞（活K562）的实验。在活K562与死K562比例为5:1的情况下，该系统能够以95%的分离效率分离目标细胞。
