Sitnik O, Jawor P, Kopeć W, Skiba T, Stefaniak T
Department of Immunology, Pathophysiology and Veterinary Prevention Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wrocław, Poland.
Pol J Vet Sci. 2013;16(2):283-91. doi: 10.2478/pjvs-2013-0039.
Aim of the study was to evaluate the effect of immunization of hens with bovine vaccines (C, R, T) on the course of IgY antibodies production against selected bovine E. coli strains, rota- and coronaviruses in egg yolk in farm conditions. The hens (40 individuals per group) were vaccinated twice, subcutaneously in four week interval and eggs were harvested once a week. Control group consisted of eggs sampled from non-vaccinated hens located in neighbouring cages. The antibody activity was measured by ELISA. All used vaccines induced the rise of IgY antibody in egg yolks. Based on the duration and the highest level of IgY antibody against bovine alimentary tract pathogens C vaccine was further used in next two trials for vaccination of 1000 hens each time. Double immunization seems to be enough in mounting response against examined pathogens for several weeks. Immunization with C vaccine allowed to harvest eggs with satisfactory levels of E. coli, rotavirus and coronavirus IgY antibodies which may be used to evaluate their protective effect by oral administration in calves.
本研究的目的是评估在农场条件下，用牛用疫苗（C、R、T）免疫母鸡对蛋黄中针对所选牛源大肠杆菌菌株、轮状病毒和冠状病毒的IgY抗体产生过程的影响。将母鸡（每组40只）进行两次皮下接种，间隔四周，每周收集一次鸡蛋。对照组由来自相邻笼子中未接种疫苗的母鸡所产的鸡蛋组成。通过ELISA法测定抗体活性。所有使用的疫苗均诱导蛋黄中IgY抗体升高。基于针对牛消化道病原体的IgY抗体的持续时间和最高水平，C疫苗在接下来的两项试验中每次用于1000只母鸡的接种。两次免疫似乎足以在数周内增强针对所检测病原体的反应。用C疫苗免疫可收获含有令人满意水平的大肠杆菌、轮状病毒和冠状病毒IgY抗体的鸡蛋，这些鸡蛋可用于通过口服给药评估其对犊牛的保护作用。
