Troncoso J C, March J L, Häner M, Aebi U
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
J Struct Biol. 1990 Mar;103(1):2-12. doi: 10.1016/1047-8477(90)90080-v.
Using electron microscopy (EM) of negatively stained samples, we have systematically explored the effect of aluminum and other multivalent cations on neurofilaments (NFs) in vitro. Interactions of these cations were investigated with bovine, rabbit, and rat spinal cord native NFs, and with 10-nm filaments reconstituted from the 68-kDa subunit (NF-L) isolated from bovine spinal cord. Our results indicated that, as has been observed with other classes of intermediate filaments (IFs), all multivalent cations caused significant aggregation of native NFs, suggesting that this phenomenon is a rather general one and not limited to aluminum. In addition, all cations tried caused significant lateral aggregation of filaments reconstituted from NF-L. Aluminum lactate had an identical effect on bovine, rabbit, and rat NFs. Because aluminum causes strong aggregation of NFs in vitro, a similar phenomenon may occur in vivo leading to the observed accumulation of NFs in neuronal perikarya of rabbits after intoxication with aluminum. These in vitro observations support the concept that some human neurological diseases characterized by the accumulation of NFs may be related to abnormal levels of multivalent cations.
利用负染样品的电子显微镜（EM）技术，我们系统地研究了铝和其他多价阳离子在体外对神经丝（NFs）的影响。研究了这些阳离子与牛、兔和大鼠脊髓天然NFs以及从牛脊髓分离的68 kDa亚基（NF-L）重构的10 nm细丝之间的相互作用。我们的结果表明，正如在其他类型的中间丝（IFs）中所观察到的那样，所有多价阳离子都会导致天然NFs的显著聚集，这表明这种现象相当普遍，并不局限于铝。此外，所有测试的阳离子都会导致由NF-L重构的细丝发生显著的侧向聚集。乳酸铝对牛、兔和大鼠的NFs具有相同的作用。由于铝在体外会导致NFs的强烈聚集，因此体内可能会出现类似现象，导致铝中毒后兔子神经元胞体中观察到的NFs积累。这些体外观察结果支持了这样一种观点，即一些以NFs积累为特征的人类神经疾病可能与多价阳离子水平异常有关。
