Tkacheva T I, Kirzon S S, Khodarev N N, Botrin I I
Biull Eksp Biol Med. 1990 Jun;109(6):543-6.
Ca, Mg-dependent endonuclease is one of the main DNAses of lymphocyte chromatin. It's activity is known to increase in the immune response and to decrease in spontaneous and experimental CLL. These observations became a basis for analysis of possible clinical meaning of it's enzymatic activity assay. Donors' peripheral blood lymphocytes being tested, normal level of endonucleolysis for men and children was defined. Except that patients with different clinical forms of lymphoproliferative diseases such as chronic lympholeukemia, non-Hodgkin lymphomas, Hodgkin's disease were observed. The results showed that Ca, Mg-dependent endonucleolysis activity was decreased in comparison to donors' one. Ca, Mg-dependent endonucleolysis activity was the same in the group of patients with non-malignant pathology and in donors' one. Successful treatment and remission state of our patients was associated with alteration of the Ca, Mg-dependent endonucleolysis activity to normal level as well as immunological parameters. That is why the activity of Ca, Mg-dependent endonucleolysis is suggested to be a new criterion of immune state and lymphocyte malignant transformation.
钙、镁依赖性核酸内切酶是淋巴细胞染色质的主要脱氧核糖核酸酶之一。已知其活性在免疫反应中增加，而在自发性和实验性慢性淋巴细胞白血病中降低。这些观察结果成为分析其酶活性测定可能的临床意义的基础。对供体的外周血淋巴细胞进行检测，确定了男性和儿童的核酸内切水平正常。除此之外，还观察了患有不同临床形式淋巴增生性疾病的患者，如慢性淋巴细胞白血病、非霍奇金淋巴瘤、霍奇金病。结果显示，与供体相比，钙、镁依赖性核酸内切活性降低。非恶性病变患者组的钙、镁依赖性核酸内切活性与供体相同。我们患者的成功治疗和缓解状态与钙、镁依赖性核酸内切活性恢复到正常水平以及免疫参数的改变有关。因此，钙、镁依赖性核酸内切活性被认为是免疫状态和淋巴细胞恶性转化的一个新指标。
