Department of Biochemistry, Institute of Veterinary Preventive Medicine, Ranipet 632402, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Dec;116:331-8. doi: 10.1016/j.saa.2013.07.038. Epub 2013 Jul 27.
In the present investigation, functionalization of gold nanoparticles synthesized using propanoic acid 2-(3-acetoxy-4,4,14-trimethylandrost-8-en-17-yl) (PAT) an active biocomponent isolated from Cassia auriculata is studied in detail. On reaction of PAT with aqueous HAuCl4, rapid formation of stable gold nanoparticles was achieved. Formation of gold nanoparticles was confirmed by UV-vis spectroscopy, XRD, GC-MS,FTIR, TEM and SEM with EDAX. Gold nanoparticles mostly were monodisperse, spherical in shape and ranged in size 12-41 nm. Gold nanoparticles synthesised using PAT was administered to alloxan (150 mg/kg body weight) induced diabetic male albino rats at different doses (0.25, 0.5, 0.75 and 1.0mg/kg body weight) for 28 days. Plasma glucose level, cholesterol and triglyceride were significantly (p<0.001) reduced in experimental animals treated with gold nanoparticles at dosage of 0.5mg/kg body weight and plasma insulin increased significantly. The newly genre green gold nanoparticles exhibit remarkable protein tyrosine phosphatase 1B inhibitory activity.
在本研究中，详细研究了使用从Cassia auriculata 中分离出的活性生物成分 2-（3-乙酰氧基-4,4,14-三甲基雄甾-8-烯-17-基）丙酸（PAT）合成的金纳米粒子的功能化。PAT 与水合 HAuCl4 反应时，可迅速形成稳定的金纳米粒子。通过紫外可见光谱，XRD，GC-MS，FTIR，TEM 和 EDAX 证实了金纳米粒子的形成。金纳米粒子主要为单分散的，球形，尺寸为 12-41nm。用 PAT 合成的金纳米粒子以不同剂量（0.25、0.5、0.75 和 1.0mg/kg 体重）施用于链脲佐菌素（150mg/kg 体重）诱导的糖尿病雄性白化大鼠，共 28 天。用 0.5mg/kg 体重的金纳米粒子处理的实验动物的血浆葡萄糖水平，胆固醇和甘油三酯显著（p<0.001）降低，血浆胰岛素显著增加。新型绿色金纳米粒子表现出显著的蛋白酪氨酸磷酸酶 1B 抑制活性。
