Alghuthaymi Mousa A, Rajkuberan Chandrasekaran, Santhiya Thiyagaraj, Krejcar Ondrej, Kuča Kamil, Periakaruppan Rajiv, Prabukumar Seetharaman
Biology Department, Science and Humanities College, Shaqra University, Alquwayiyah 19245, Saudi Arabia.
Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore 641021, India.
Plants (Basel). 2021 Nov 3;10(11):2370. doi: 10.3390/plants10112370.
The developments of green-based metallic nanoparticles (gold) are gaining tremendous interest, having potential applications in health care and diagnosis. Therefore, in the present study, flower filtered extract was used as a reducing and stabilizing agent to synthesize gold nanoparticles (PtubAuNPs). The PtubAuNPs were extensively characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and X-ray diffraction. The antibacterial activity of PtubAuNPs was determined by the agar well diffusion method; the PtubAuNPs performed extreme antagonistic activity against the tested pathogens. Furthermore, the cytotoxicity of the PtubAuNPs was evaluated in MCF 7 cells by MTT assay. The PtubAuNPs induced toxicity in MCF 7 cells with the least concentration of 100 µg/mL in a dose-dependent method by inducing apoptosis. Overall, the study manifested that PtubAuNPs are a potent nanomaterial that can be employed as an antimicrobial and anticancer agent.
基于绿色的金属纳米颗粒(金)的发展正引起人们极大的兴趣,在医疗保健和诊断方面具有潜在应用。因此,在本研究中,花过滤提取物被用作还原剂和稳定剂来合成金纳米颗粒(PtubAuNPs)。通过紫外可见光谱、傅里叶变换红外光谱、透射电子显微镜和X射线衍射对PtubAuNPs进行了广泛表征。采用琼脂孔扩散法测定了PtubAuNPs的抗菌活性;PtubAuNPs对受试病原体表现出极强的拮抗活性。此外,通过MTT法在MCF 7细胞中评估了PtubAuNPs的细胞毒性。PtubAuNPs通过诱导凋亡以剂量依赖方式在最低浓度为100 µg/mL时诱导MCF 7细胞毒性。总体而言,该研究表明PtubAuNPs是一种有效的纳米材料,可作为抗菌和抗癌剂使用。