School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Dec;116:440-5. doi: 10.1016/j.saa.2013.07.078. Epub 2013 Aug 6.
A fast and sensitive fluorometric method for determination of an emerging pollutant, ethylenediaminetetraacetic acid (EDTA), was proposed based on the reversion of fluorescence quenching of 2-pyridinecarbaldehyde-p-phenyldihydrazone by ferric iron. It was found that Fe(3+) could selectively quench the fluorescence of 2PC-PPH, while EDTA could recover it due to its strong coordination ability with Fe(3+). Based on this, we established an "Off and On" fluorescence method for determination of EDTA. Under the optimum conditions, the linear range and detection limit (3S/N) for EDTA determination were 2.0 × 10(-6)~1.4 × 10(-5) mol L(-1) and 1.5 × 10(-7) mol L(-1), respectively. The relative standard deviation for the determination of 7.0 × 10(-6)mol L(-1) EDTA was 3.7% (n=11). The method was successfully applied to determination of EDTA in tap water, lake water and investigating degradation of EDTA by TiO2/UV process.
一种快速灵敏的荧光测定法用于测定一种新兴污染物——乙二胺四乙酸（EDTA）。该方法基于 2-吡啶醛缩对苯二肼（2PC-PPH）的荧光猝灭被三价铁离子恢复这一原理。研究发现，Fe(3+) 可以选择性地猝灭 2PC-PPH 的荧光，而 EDTA 由于其与 Fe(3+) 的强配位能力可以恢复其荧光。基于这一点，我们建立了一种用于测定 EDTA 的“关-开”荧光方法。在最佳条件下，EDTA 的线性范围和检测限（3S/N）分别为 2.0×10(-6)~1.4×10(-5)mol L(-1)和 1.5×10(-7)mol L(-1)。测定 7.0×10(-6)mol L(-1) EDTA 的相对标准偏差为 3.7%（n=11）。该方法成功应用于自来水中 EDTA 的测定以及研究 TiO2/UV 工艺对 EDTA 的降解。
