Gross-Isseroff R, Salama D, Israeli M, Biegon A
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Brain Res. 1990 Jun 11;519(1-2):223-7. doi: 10.1016/0006-8993(90)90081-l.
Autoradiographic analysis of 5-HT2 receptors in the human brain, using [3H]ketanserin as a ligand, reveals region-specific changes in receptor labeling as a function of age. In the prefrontal cortex and hippocampal dentate gyrus of 12 normal subjects, label density decreases sharply with age over the 2nd and 3rd decades, reaches a minimum around age 50 and then starts to increase again in the 6th and 7th decades. Other brain regions studied, including frontoparietal and temporal cortex, basal ganglia and thalamus, did not show significant changes with age. Saturation binding experiments on prefrontal cortical samples from 23 normal subjects reveal that the decrease in label density is due to changes in receptor density (Bmax) with no apparent change in affinity (Kd). Sex, presence of alcohol and postmortem delay had no effect on ketanserin binding.
利用[³H]酮色林作为配体对人类大脑中的5-羟色胺2（5-HT2）受体进行放射自显影分析，结果显示受体标记的区域特异性变化是年龄的函数。在12名正常受试者的前额叶皮质和海马齿状回中，标记密度在第二和第三个十年随年龄急剧下降，在50岁左右达到最低，然后在第六和第七个十年又开始增加。研究的其他脑区，包括额顶叶和颞叶皮质、基底神经节和丘脑，未显示出随年龄的显著变化。对23名正常受试者前额叶皮质样本进行的饱和结合实验表明，标记密度的降低是由于受体密度（Bmax）的变化，而亲和力（Kd）没有明显变化。性别、是否饮酒和死后延迟对酮色林结合没有影响。
