Gross-Isseroff R, Salama D, Israeli M, Biegon A
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Brain Res. 1990 Jan 22;507(2):208-15. doi: 10.1016/0006-8993(90)90274-f.
In vitro quantitative autoradiography of 5-HT2 receptors, using [3H]ketanserin as a ligand, was performed on 24 human brains postmortem. Twelve brains were donated by suicide victims and 12 by matched controls. We found a characteristic decline in 5-HT2 receptors with age in several brain regions of the control group. This age dependence of ketanserin binding was not present in some of these brain regions of the suicide group. We also found a significant but anatomically selective reduction in the density of ketanserin binding sites in the young suicide group, compared to age-matched controls. This reduction was evident in portions of the prefrontal cortex. Homogenate binding assays on prefrontal cortex samples from a large group of suicides (n = 20) and controls (n = 23) showed that the difference in age dependence of ketanserin binding and the reduced binding in the young suicide group were explained by differences in Bmax values. No differences were observed in Kd. Sex, presence of alcohol and time from death to autopsy did not affect ketanserin binding, in our sample, as measured by either autoradiography or homogenate binding assay.
使用[³H]酮色林作为配体，对24例人类死后大脑进行了5-HT₂受体的体外定量放射自显影。12个大脑由自杀受害者捐赠，12个由匹配的对照组捐赠。我们发现对照组的几个脑区中，5-HT₂受体随年龄呈现特征性下降。自杀组的某些脑区不存在酮色林结合的这种年龄依赖性。我们还发现，与年龄匹配的对照组相比，年轻自杀组中酮色林结合位点密度有显著但具有解剖学选择性的降低。这种降低在前额叶皮质部分很明显。对一大组自杀者（n = 20）和对照组（n = 23）的前额叶皮质样本进行的匀浆结合测定表明，酮色林结合的年龄依赖性差异以及年轻自杀组中结合减少是由Bmax值的差异所解释的。未观察到Kd有差异。在我们的样本中，通过放射自显影或匀浆结合测定，性别、酒精的存在以及从死亡到尸检的时间均未影响酮色林结合。
