大肠杆菌核糖体中与肽基转移酶的肽基识别中心相邻的核糖核酸成分的亲和标记
Affinity labeling of the ribonucleic acid component adjacent to the peptidyl recognition center of peptidyl transferase in Escherichia coli ribosomes.
作者信息
Yukioka M, Hatayama T, Morisawa S
出版信息
Biochim Biophys Acta. 1975 May 1;390(2):192-208. doi: 10.1016/0005-2787(75)90341-x.
N-Iodacetylphenylalanyl-tRNA was used as an affinity label for localizing the RNA components intimately related to the peptidyl transferase activity of Escherichia coli ribosomesmthis analogue could specifically alkylate a unique nucleotide chain of 23-S RNA. The alkylation was strongly enhanced by poly(U), and was dependent on the presence of both 50- and 30-S subunits; Chloramphenicol inhibited the reaction, wheras blasticidin S stimulated it. The alkylated RNA base was found to be adenine. The nucleotide chain attacked by N-iodoacetylphenylalanyl-tRNA seemed to be localized at or near to the peptidyl recognition center of peptidyl transferase.
N-碘乙酰苯丙氨酰-tRNA被用作亲和标记物，以定位与大肠杆菌核糖体肽基转移酶活性密切相关的RNA成分。这种类似物可以特异性地烷基化23-S RNA的一条独特核苷酸链。聚尿苷酸（poly(U)）强烈增强了烷基化反应，并且该反应依赖于50-S和30-S亚基的同时存在；氯霉素抑制该反应，而杀稻瘟菌素S则刺激该反应。发现被烷基化的RNA碱基是腺嘌呤。被N-碘乙酰苯丙氨酰-tRNA攻击的核苷酸链似乎定位在肽基转移酶的肽基识别中心处或其附近。
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