Cao Zhi, Wang Yang, Liu Zhi-Yong, Zhang Zhen-Sheng, Ren Shan-Cheng, Yu Yong-Wei, Qiao Meng, Zhai Bei-Bei, Sun Ying-Hao
Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Asian J Androl. 2013 Nov;15(6):742-6. doi: 10.1038/aja.2013.79. Epub 2013 Aug 26.
Transglutaminase 4 has been shown to enhance various biological properties of prostate cancer cells, e.g., cell-matrix adhesion, invasiveness and the epithelial-mesenchymal transition. The objectives of this study were to investigate the associations between transglutaminase 4 expression and the established features and biochemical recurrence of prostate cancer. Transglutaminase 4 immunostaining was performed on a tissue microarray. The expression of transglutaminase 4 was evaluated by a scoring method based on the intensity and extent of staining. The clinical and pathological information was obtained through a review of medical records. Follow-up data were obtained by consulting the hospital medical records and the prostate cancer database of our department and by contacting patients or family members. We then compared the transglutaminase 4 expression levels between the prostate cancer tissues and the paracarcinoma tissues and evaluated the correlation of transglutaminase 4 expression with the clinical parameters and biochemical recurrence of prostate cancer. Our results indicated that the transglutaminase 4 staining was significantly higher in tumour tissue than in paracarcinoma tissue (P<0.001) and was positively associated with higher Gleason score (P<0.001) and higher prostate-specific antigen level (P=0.005). Patients with transglutaminase 4 overexpression experienced shorter biochemical recurrence-free survival after surgery (P=0.042) in the univariate analysis but not in the multivariate analysis (P=0.139), which indicated that transglutaminase 4 may serve as a potential predictor of biochemical recurrence of prostate cancer.
转谷氨酰胺酶4已被证明可增强前列腺癌细胞的多种生物学特性，例如细胞与基质的黏附、侵袭性以及上皮-间质转化。本研究的目的是调查转谷氨酰胺酶4表达与前列腺癌既定特征及生化复发之间的关联。在组织芯片上进行转谷氨酰胺酶4免疫染色。通过基于染色强度和范围的评分方法评估转谷氨酰胺酶4的表达。通过查阅病历获取临床和病理信息。通过查阅我院病历和科室前列腺癌数据库以及联系患者或家属获得随访数据。然后我们比较了前列腺癌组织和癌旁组织中转谷氨酰胺酶4的表达水平，并评估了转谷氨酰胺酶4表达与前列腺癌临床参数及生化复发的相关性。我们的结果表明，肿瘤组织中转谷氨酰胺酶4染色显著高于癌旁组织（P<0.001），且与较高的Gleason评分（P<0.001）和较高的前列腺特异性抗原水平呈正相关（P=0.005）。在单因素分析中转谷氨酰胺酶4过表达的患者术后无生化复发生存期较短（P=0.042），但在多因素分析中并非如此（P=0.139），这表明转谷氨酰胺酶4可能作为前列腺癌生化复发的潜在预测指标。
