Aoyagi Hidekazu, Iwasaki Shin-Ichi, Nakamura Kenzirou
Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-chou, Chuo-ku, Niigata, 951-8580, Japan,
Odontology. 2015 Jan;103(1):1-8. doi: 10.1007/s10266-013-0131-4. Epub 2013 Aug 22.
The aim of this study is to obtain information about the mouse tongue muscle rendered using micro-computed tomography (μCT) at low, middle, and high magnifications. Three-dimensional (3D) μCT is used in various fields. Most μCT observations are restricted to hard tissue in biomaterial samples. Recently, with the use of osmium tetroxide, μCT has been effectively employed to observe soft tissue; it is now believed that μCT observation of soft tissue is feasible. On the other hand, the structure of the tongue muscle has been well studied, but cross-sectional imaging enhanced by 3D rendering is lacking. We chose the mouse tongue as a soft tissue case study for μCT and generated cross-sectional images of the tongue enhanced by 3-D image rendering with histological resolution. During this observation, we developed new methods of low-magnification observation to show the relation between the tongue muscles and surrounding tissues. We also applied high-resolution μCT in high-magnification observation of muscle fiber fascicles. Our methodological techniques give the following results: (1) For low-magnification observation (field of view: 12,000 μm), pretreatment with decalcification and freeze drying is suitable for observing the area between the muscle of the tongue and the bone around the tongue using μCT. (2) For middle-magnification observation (Field of view: 3,500 μm), the use of osmium tetroxide to observe the muscle arrangement of the tongue by μCT is suitable. (3) For high-magnification observation (Field of view: 450 μm), high-resolution μCT is suitable for observation of the transversus muscle fiber fascicles.
本研究的目的是获取有关使用微计算机断层扫描（μCT）在低、中、高放大倍数下呈现的小鼠舌肌的信息。三维（3D）μCT在各个领域都有应用。大多数μCT观察仅限于生物材料样本中的硬组织。最近，通过使用四氧化锇，μCT已有效地用于观察软组织；现在人们认为对软组织进行μCT观察是可行的。另一方面，舌肌的结构已经得到了充分研究，但缺乏通过3D渲染增强的横截面成像。我们选择小鼠舌作为μCT软组织案例研究对象，并生成了具有组织学分辨率的3D图像渲染增强的舌横截面图像。在这个观察过程中，我们开发了低倍观察的新方法，以显示舌肌与周围组织之间的关系。我们还将高分辨率μCT应用于肌纤维束的高倍观察。我们的方法技术得出以下结果：（1）对于低倍观察（视野：12,000μm），脱钙和冷冻干燥预处理适用于使用μCT观察舌肌与舌周围骨骼之间的区域。（2）对于中倍观察（视野：3,500μm），使用四氧化锇通过μCT观察舌的肌肉排列是合适的。（3）对于高倍观察（视野：450μm），高分辨率μCT适用于观察横肌纤维束。
