Advanced Research Center, School of Life Dentistry at Niigata, Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
Odontology. 2010 Feb;98(1):26-30. doi: 10.1007/s10266-009-0112-9. Epub 2010 Feb 16.
The purpose of this study was to demonstrate a micro-computed tomography (CT) method for observations of the mouse embryo. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained en bloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-CT and image processing was performed. Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. The trigeminal ganglion was imaged using three-dimensional techniques. Observation of the embryo was possible by micro-CT with osmium tetroxide staining.
本研究旨在展示一种用于观察小鼠胚胎的微计算机断层扫描（CT）方法。在受精后 13.0 天，将小鼠胚胎用 4%多聚甲醛固定 24 小时，并通过锇四氧化物整夜包埋染色。然后，将胚胎用标准方法在石蜡中包埋 24 小时。通过微 CT 分析标本，并进行图像处理。由于不同的锇染色密度，可以观察到含有神经和血液系统的器官。使用三维技术对三叉神经节进行成像。通过锇四氧化物染色的微 CT 可以观察胚胎。
